IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base

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Authors

Abstract

It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter (BBa_J23101) and a strong ribosome bonding site (BBa_I13003) in Escherichia .coli to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the growth and viability of cells were not affected by strong gene expression.

Introduction

Methods and Materials

Results and Interpretations

Discussion

Conclusion