IGEM:Hong Kong HKUST/Investigations: Difference between revisions
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*[[IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning by Standard Assembly /Entry Base | Plasmid Cloning by Standard Assembly]] | *[[IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning by Standard Assembly /Entry Base | Plasmid Cloning by Standard Assembly]] | ||
*[[IGEM:Hong Kong HKUST/Investigations/ Restriction Enzyme Activities At 4°C,37°C and 65°C | Restriction Enzyme Activities At 4°C,37°C and 65°C]] | *[[IGEM:Hong Kong HKUST/Investigations/ Restriction Enzyme Activities At 4°C,37°C and 65°C | Restriction Enzyme Activities At 4°C,37°C and 65°C]] | ||
*[[IGEM:Hong Kong HKUST/Investigations/ Effect of dephosphorylation in reducing the background ligation | Effect of dephosphorylation in reducing the background ligation]] | |||
Revision as of 18:39, 1 February 2016
List of Investigations:
(Sorted by alphabetic order)
2014
- Comparison Between WN Buffer & 70% Ethanol in Viogene Miniprep Kit
- Comparison of Different Growth Media for Plasmid Production
- Effect of Agarose Concentrations on Separation of Linear DNA in Gel Electrophoresis
- Effect of ATP Concentrations on Ligation Efficiency
- Performance of NotI-HF in 5, 15 and 30 minutes
- Recovery Time and Transformation Efficiency
- Restriction Enzyme Activities at 37°C vs. 20°C
- Robustness of Colony PCR Compared to Regular PCR
2015
- Investigation on the Functionality of RNA Thermometers
- Investigation on the Light-Sensitive amilCP Generator
- Effect of overloading cells on growth rate
- Effect of Dephosphorylation on Reduction of Background Ligation
- Identifying candidate colonies in molecular level
- Effects of ATP concentration to the Ligation Efficiency
- Plasmid Cloning by Restriction Enzyme Digestion
- The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation
