IGEM:Hong Kong UST 2014/2009/Notebook/ATP Concentration Effect to Ligation Speed/2014/06/03: Difference between revisions

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==Tuesday, 3th of June 2014==
==Tuesday, 3th of June 2014==
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'''1. Preparation of Chloramphenicol(CHL) LB plates:'''
* '''Preparation of Chloramphenicol LB plates:'''
500µl of 150mg/ml of Chloramphenicol was added to 500 ml of LB broth solution.  
500µl of 150mg/ml of Chloramphenicol was added to 500 ml of LB broth solution.  
All plates were then stored in refrigerator.
All plates were then stored in refrigerator.


*'''Inoculation of bacteria in broth:'''
'''2. Inoculation of bacteria in broth:'''
E.Coli SURE with RFP-PSB1C3 was obtained from four different colonies and inoculated to the 15 ml Falcon tube with 5 ml CAM LB broth. The tubes were incubated under 37ºC for 16 hours, 200 rpm.
''E.coli'' SURE with RFP-PSB1C3 was obtained from four different colonies and inoculated to the 15 ml Falcon tube with 5 ml CAM LB broth. The tubes were incubated under 37ºC for 16 hours, 200 rpm.
The inoculated bacteria were named RFP-PSB1C3 1-4.
The inoculated bacteria were named RFP-pSB1C3 1-4.<br><br>
 
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Revision as of 08:16, 9 June 2014

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Tuesday, 3th of June 2014

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1. Preparation of Chloramphenicol(CHL) LB plates: 500µl of 150mg/ml of Chloramphenicol was added to 500 ml of LB broth solution. All plates were then stored in refrigerator.

2. Inoculation of bacteria in broth: E.coli SURE with RFP-PSB1C3 was obtained from four different colonies and inoculated to the 15 ml Falcon tube with 5 ml CAM LB broth. The tubes were incubated under 37ºC for 16 hours, 200 rpm. The inoculated bacteria were named RFP-pSB1C3 1-4.

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