IGEM:Hong Kong UST 2014/2009/Notebook/ATP Concentration Effect to Ligation Speed/2014/06/04: Difference between revisions
No edit summary |
No edit summary |
||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
==Wednesday, 4th June 2014== | ==Wednesday, 4th June 2014== | ||
<html><img src="http://openwetware.org/images/2/26/Team_copy.jpg" width="20%" align="right"></html><br> | |||
<html><img src="http://openwetware.org/images/8/8d/Procedure-2_copy.jpg" width="65%"></html><br> | <html><img src="http://openwetware.org/images/8/8d/Procedure-2_copy.jpg" width="65%"></html><br> | ||
Revision as of 23:37, 7 June 2014
ATP Concentration Effect to Ligation Speed | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Wednesday, 4th June 2014<html><img src="http://openwetware.org/images/2/26/Team_copy.jpg" width="20%" align="right"></html>
Plasmids were extracted using Gene Tech GTpure Plasmid Miniprep Purification Kit. DNA then eluted in 50 μL DE Buffer (2 times)
Use DE Buffer as Blank
EcoRI-HF & PstI-HF
0.8% Agarose Gel, 20 ml, 6 wells WELL 1 (LADDER): WELL 2 (NEGATIVE CONTROL):
- 1 uL of Uncut Plasmid RFP-PSB1C3(2) (124.8 ng/lane) WELL 3-6 (DIGESTION PRODUCT)(1ug/lane):
<html><img src="http://openwetware.org/images/1/16/20140606131708644.jpg" width="65%"></html> Ladder - Uncut RFP-PSB1C3 - EcoRI-HF and PstI-HF digested RFP-PSB1C3 (1-4)
Insert and backbone were extracted out from the gel using Favorgen FavorPrep GEL/PCR Purification Kit. The DNA is then eluted in 50 μL of Elution Buffer
|