IGEM:IMPERIAL/2006/LabCalendar/2006-7-18: Difference between revisions
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== | ==Identification and extraction of parts== | ||
We have already successfully extracted and cultured 4 parts (All from Plate 1) | |||
*I13272 well 4F | |||
*F2621 well 4N | |||
*I13273 well 4H | |||
*I13208 well 4J | |||
A further 16 parts were identified as being necessary for use in our project. (From Plate 1 unless stated) | |||
*F2620 well 6B | *F2620 well 6B | ||
*I0460 well 11I | *I0460 well 11I | ||
*F1610 well 1B | *F1610 well 1B (Plate 2) | ||
*E0040 well 5H | *E0040 well 5H | ||
*T9002 well 19B | *T9002 well 19B (Plate 2) | ||
*I13207 well 2P | *I13207 well 2P | ||
*I0403 well 5P | *I0403 well 5P | ||
| Line 20: | Line 30: | ||
*C0062 well 7A | *C0062 well 7A | ||
*I13604 well 12D | *I13604 well 12D | ||
*I14032 well 17K (Kanomycin resistant plasmid, cultured in Kanomycin plate) | *I14032 well 17K (Plate 2) (Kanomycin resistant plasmid, cultured in Kanomycin plate) | ||
*C0260 well 24C | *C0260 well 24C | ||
*C0160 well 9F | *C0160 well 9F | ||
==Electroporation== | |||
Preformed electroporation on competent cells with the following plasmids and set in 37C incubator overnight to grow. All except 17K were incubated on ampicillin plates since they all had ampicillin resistance gene. | |||
Revision as of 02:02, 20 July 2006
Identification and extraction of parts
We have already successfully extracted and cultured 4 parts (All from Plate 1)
- I13272 well 4F
- F2621 well 4N
- I13273 well 4H
- I13208 well 4J
A further 16 parts were identified as being necessary for use in our project. (From Plate 1 unless stated)
- F2620 well 6B
- I0460 well 11I
- F1610 well 1B (Plate 2)
- E0040 well 5H
- T9002 well 19B (Plate 2)
- I13207 well 2P
- I0403 well 5P
- C0261 well 24A
- E0434 well 20J
- R0062 well 9G
- B0034 well 3O
- C0062 well 7A
- I13604 well 12D
- I14032 well 17K (Plate 2) (Kanomycin resistant plasmid, cultured in Kanomycin plate)
- C0260 well 24C
- C0160 well 9F
Electroporation
Preformed electroporation on competent cells with the following plasmids and set in 37C incubator overnight to grow. All except 17K were incubated on ampicillin plates since they all had ampicillin resistance gene.
