IGEM:IMPERIAL/2006/LabCalendar/2006-7-26
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For Maxiprep
- F2620 well 6B
- C0261 well 24A
- I13504 well 12D
Transferred to 2mL LB liquid culture in preparation for Maxiprep - in 37C shaker
For Miniprep
- I13033 well 2H
- J04500 well 16P
- C0060 well 5M
Above transfereed to 2mL LB liquid culture in preparation for miniprep - in 37C shaker
- R0062 well 9G
- B0034 well 3O
- C0062 well 7A
Above transferred to 2 mL LB liquid culture in preparation for miniprep. Separately, from previous miniprep, took 10 uL out to digest and to run gels later..
- Made up 1% agarose solution, 60 mL ready for 3 gel plates, 2 mL ethidium bromide, and set to cool
- Added TAE to prevent from drying out
- A/O 12pm, the three digests are in 37C water bath before moving to gels in an hour
Prepare the following parts for Maxiprep and ligations for tomorrow
- I13033 well 2H
- C0062 well 7A
- F2620 well 6B
- C0261 well 24A
- R0062 well 9G
- B0034 well 3O
- I13504 well 12D
Culture 100 mL LB and 200 uL bacteria with part in 500 mL flask. Set overnight in 37C shaker to culture.
