IGEM:IMPERIAL/2006/LabCalendar/2006-8-29

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Revision as of 04:22, 29 August 2006 by Cys (talk | contribs) (→‎To Do:)
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To Do:

*Culture up the ligated parts (J37022 & J37025) for miniprep (to check whether ligations from Monday 
 were successful) from plates in incubator 
*Make frozen stock of 4G(S01656) and J37018
*Make more 50 times TAE
*Culture for testing: T9002, J37015, J37020
*To order: LB medium (or make up ourselves & send to autoclave) & make some LB Amp to have in stock 
           Autoclaved flasks (and cuvettes?)
           Need more eppendorfs
           More LB Amp plates ? (How many more do we need for our remaining time - there are about 10 left)
*Gels from the weekend need to be analysed: Were J37018 & J37024 ligations successful ?


LoxP PCR

  • Run once more, this time with DMSO, and a larger amount of maxiprepped DNA
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