IGEM:IMPERIAL/2006/LabCalendar/2006-8-30: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 6: Line 6:
<font color="#FF8000">
<font color="#FF8000">
===To Do:===
===To Do:===
  *Maxiprep (for new ligations) & Miniprep (to check whether ligations from Monday were successful)
  *Maxiprep of J37022 and J37025 & Miniprep (to check whether ligations from Monday were successful)
   Note: If Miniprep shows successful ligation, J27022 & J37025 need to be sent for sequencing (PRIMERS !!)
   Note: If Miniprep shows successful ligation, J27022 & J37025 need to be sent for sequencing (PRIMERS !!)
  *Ligation & Electroporation:
  *Make a stock of frozen cells of J37022 and J37025 directly from that overnight culture
          - J37025 = B0034 + J37024
          - J37022 = J04500 + J37024


</font color>
</font color>

Revision as of 16:04, 28 August 2006



To Do:

*Maxiprep of J37022 and J37025 & Miniprep (to check whether ligations from Monday were successful)
 Note: If Miniprep shows successful ligation, J27022 & J37025 need to be sent for sequencing (PRIMERS !!)
*Make a stock of frozen cells of J37022 and J37025 directly from that overnight culture