IGEM:IMPERIAL/2006/LabCalendar/2006-8-31: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 19: Line 19:


Put in shaker at 9:40. To be taken out at 11:40.
Put in shaker at 9:40. To be taken out at 11:40.
''After 2hrs''
*OD of T9002: 0.827        (innoculate 3.023ml culture into 16ml)
*OD of J37016: 0.947        (innoculate 2.640ml culture into 16ml)
*OD of J37020: 0.806        (innoculate 3.102ml culture into 16ml)
Put in the shaker at 12:10. To be taken out at 4:10.

Revision as of 04:26, 31 August 2006



TO DO:
*Testing of T9002, J37016, J37020, S01656, J37015, J37015RS
*Culture new ligated part J37024 for mini & maxiprep on Friday
*Miniprep 'old' J37024 (both plates)


Make up test media dilutions for the chemostat. I will make a dilution series of diferent LB concentrations (keeping salts the same) to get a lower OD, idealy around 0.3 / 0.4 Then tomorrow I will use glycerol to increace the nutriant concentration of the media so the LB becomes carbon limiting, trying to get a glycerol conc of 0.8. Today I will also make media (LB + Glycerol that can support a population up to an OD of >2.8 JohnChattaway 04:25, 31 August 2006 (EDT)

Testing T9002/J37016/J37015

  • OD of o/n T9002 culture: 2.00
  • OD of o/n J37016 culture: 2.00
  • OD of o/n J37020 culture: 2.00

Put in shaker at 9:40. To be taken out at 11:40.

After 2hrs

  • OD of T9002: 0.827 (innoculate 3.023ml culture into 16ml)
  • OD of J37016: 0.947 (innoculate 2.640ml culture into 16ml)
  • OD of J37020: 0.806 (innoculate 3.102ml culture into 16ml)

Put in the shaker at 12:10. To be taken out at 4:10.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:IMPERIAL/2006/LabCalendar/2006-8-31&oldid=66162"