IGEM:IMPERIAL/2006/LabCalendar/2006-8-31

• IGEM:IMPERIAL/2006/LabCalendar/2006-8-31 Events
• Day in the lab
• Project Progress

TO DO:
*Testing of T9002, J37016, J37020, S01656, J37015, J37015RS
*Culture new ligated part J37024 for mini & maxiprep on Friday
*Miniprep 'old' J37024 (both plates)

Make up test media dilutions for the chemostat. I will make a dilution series of diferent LB concentrations (keeping salts the same) to get a lower OD, idealy around 0.3 / 0.4 Then tomorrow I will use glycerol to increace the nutriant concentration of the media so the LB becomes carbon limiting, trying to get a glycerol conc of 0.8. Today I will also make media (LB + Glycerol that can support a population up to an OD of >2.8 JohnChattaway 04:25, 31 August 2006 (EDT)

Testing T9002/J37016/J37015

• OD of o/n T9002 culture: 2.00
• OD of o/n J37016 culture: 2.00
• OD of o/n J37020 culture: 2.00

Put in shaker at 9:40. To be taken out at 11:40.

After 2hrs

• OD of T9002: 0.827 (innoculate 3.023ml culture into 16ml)
• OD of J37016: 0.947 (innoculate 2.640ml culture into 16ml)
• OD of J37020: 0.806 (innoculate 3.102ml culture into 16ml)

Put in the shaker at 12:10. To be taken out at 4:10.

Miniprep

• 4 J37024 1 samples
• 4 J37024 2 samples
• 3 J37018 samples

See here for gel results

Culturing

• Culture up the repeated ligation of j37024 (II) to maxi and mini tomorrow.

To do tomorrow

• Maxiprep J37024 II
• Ligate to form J37025
• Re ligate to form part J37018