IGEM:IMPERIAL/2006/LabCalendar/2006-8-8: Difference between revisions
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Deepti and Jo<font color="red">'''h'''</font color="red">nny cultured up A with the last of the LB so it should be ready to maxiprep for tomorrow. The other ligations have a good chance of having been maxipreped successfuly so we can decide what to do tomorrow (LBs arriving tomorrow anyway). | Deepti and Jo<font color="red">'''h'''</font color="red">nny cultured up A with the last of the LB so it should be ready to maxiprep for tomorrow. The other ligations have a good chance of having been maxipreped successfuly so we can decide what to do tomorrow (LBs arriving tomorrow anyway). | ||
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Below is a picture of the gels showing results of both, minipreps and ligations yesterday and today. Still have to put up the riboswitch gels. | Below is a picture of the [http://openwetware.org/images/b/b0/Pcr-miniprep_gels_stage_2_ligations.pdf| gels] showing results of both, minipreps and ligations yesterday and today. Still have to put up the riboswitch gels. | ||
Revision as of 09:00, 8 August 2006
Electroporation of Ligations from yesterday
- Electroporated 12D->2H (3rd attempt...should be successful??? thrid time's a charm?) and 12D->2H B (the big part from the gel)
- Plates are in the incubator, needs to be minipreped tomorrow to check...and probably PCR to correlate.
Maxiprep of Ligations from yesterday
- Results of maxiprep of ligations A, M and Z may be dubious. Should repeat maxiprep for assurance.
- Maxiprep of ligations L, R and V should be fine.
To ensure we can re-maxiprep tomorrow we need to culture up more bacteria by the end of today.
Deepti and Johnny cultured up A with the last of the LB so it should be ready to maxiprep for tomorrow. The other ligations have a good chance of having been maxipreped successfuly so we can decide what to do tomorrow (LBs arriving tomorrow anyway).
Below is a picture of the gels showing results of both, minipreps and ligations yesterday and today. Still have to put up the riboswitch gels.
