IGEM:IMPERIAL/2006/LabCalendar/2006-9-1: Difference between revisions
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*Put in the shaker at 9.25. Take out at 11.25. | *Put in the shaker at 9.25. Take out at 11.25. | ||
After 2 hrs: | |||
*Took OD of cultures, innoculated into 25ml, placed in shaker at 11:45. To be taken out at 3:45. | |||
==Maxiprep== | ==Maxiprep== |
Revision as of 05:56, 1 September 2006
TO DO: *Testing of T9002, J37016, J37020, S01656(contd. from Thursday), J37015, J37015RS *Mini & Maxiprep of new J37024 (CHECK the miniprep gel straight after !) *Ligation of J37022, J37025 (using new J37024)
Testing T9002/J37016/J37020
- OD of o/n culture of T9002: 2.00
- OD of o/n culture of J37016: 2.00
- OD of o/n culture of J37020: 2.00
- Put in the shaker at 9.25. Take out at 11.25.
After 2 hrs:
- Took OD of cultures, innoculated into 25ml, placed in shaker at 11:45. To be taken out at 3:45.
Maxiprep
- Maxiprep 3 samples of J37024 II
- Throw away original maxiprep of J37024 (I) as minipreps show it to have not been successful
Ligations
- All three samples of J37024 II inserted into vectors 3O Predator (AiiA contstruct for predator cell J37025) and 16P (AiiA test construct J37022)