IGEM:IMPERIAL/2006/LabCalendar/2006-9-1

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(Testing T9002/J37016/J37020)
(Testing T9002/J37016/J37020)
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*Put in the shaker at 9.25. Take out at 11.25.
*Put in the shaker at 9.25. Take out at 11.25.
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==Maxiprep==
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*Maxiprep 3 samples of J37024 II
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*Throw away original maxiprep of J37024 (I) as minipreps show it to have not been successful
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==Ligations==
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*All three samples of J37024 II inserted into vectors 3O Predator (AiiA contstruct for predator cell J37025) and 16P (AiiA test construct J37022)

Revision as of 07:30, 1 September 2006



TO DO:
*Testing of T9002, J37016, J37020, S01656(contd. from Thursday), J37015, J37015RS
*Mini & Maxiprep of new J37024 (CHECK the miniprep gel straight after !)
*Ligation of J37022, J37025 (using new J37024)

Testing T9002/J37016/J37020

  • OD of o/n culture of T9002: 2.00
  • OD of o/n culture of J37016: 2.00
  • OD of o/n culture of J37020: 2.00
  • Put in the shaker at 9.25. Take out at 11.25.

Maxiprep

  • Maxiprep 3 samples of J37024 II
  • Throw away original maxiprep of J37024 (I) as minipreps show it to have not been successful

Ligations

  • All three samples of J37024 II inserted into vectors 3O Predator (AiiA contstruct for predator cell J37025) and 16P (AiiA test construct J37022)
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