IGEM:IMPERIAL/2006/LabCalendar/2006-9-1

• IGEM:IMPERIAL/2006/LabCalendar/2006-9-1 Events
• Day in the lab
• Project Progress

TO DO:
*Testing of T9002, J37016, J37020, S01656(contd. from Thursday), J37015, J37015RS
*Mini & Maxiprep of new J37024 (CHECK the miniprep gel straight after !)
*Ligation of J37022, J37025 (using new J37024)

Testing T9002/J37016/J37020

• OD of o/n culture of T9002: 2.00
• OD of o/n culture of J37016: 2.00
• OD of o/n culture of J37020: 2.00

• Put in the shaker at 9.25. Take out at 11.25.

After 2 hrs:

• Took OD of cultures, innoculated into 25ml, placed in shaker at 11:45. To be taken out at 3:45.

Maxiprep

• Maxiprep 3 samples of J37024 II
• Throw away original maxiprep of J37024 (I) as minipreps show it to have not been successful

Ligations

• All three samples of J37024 II inserted into vectors 3O Predator (AiiA contstruct for predator cell J37025) and 16P (AiiA test construct J37022)