IGEM:IMPERIAL/2006/LabCalendar/2006-9-12: Difference between revisions
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Revision as of 01:43, 12 September 2006
TO DO: *Maxiprep & Minprep J37025 & J37022 ? (Depends on results of J37024 control miniprep from yesterday) *Electroporate the overnight ligation of J37024 from Dr. Mann *Sequencing 5M, Aiia, (022 & 025) *Analyse the Western Blot for protein (Aiia) expression
Western blot
This didn't work. There is no diference between the controll and the test (both circled in white). However the strain used can only express one of the promoters and there was background expression reported by Dr fray, both of these could hide a positive result.
