IGEM:IMPERIAL/2006/LabCalendar/2006-9-3: Difference between revisions
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*Re-ligating J37018 | *Re-ligating J37018 | ||
*Ligating J37036 (one of the final ones...) | *Ligating J37036 (one of the final ones...) | ||
==Culturing== | |||
*Cultured up 2ml of T9002, J37015, J37015RS, J37016 and J37020 from frozen stick | |||
*Cultured up 100ml of aiiA from Dr.Frey in LB medium for maxiprep | |||
*Cultured up 2ml of 4 different J37022 colonies | |||
*Cultured up 2ml of 4 different J37025 colonies | |||
==Electroporation== | |||
*Had a bit of problem with continual blowing up, so used Kirsten's machine | |||
*Successfully electroporated J37031 + 9G | |||
*Unsuccessful for J37025 + J37019 (repeat tomorrow morning | |||
Revision as of 02:12, 4 September 2006
TO DO: *Testing of T9002, J37016, J37020, S01656, J37015, J37015RS *Mini & maxiprep of J37022 and J37025 (CHECK whether ligations were successful) *Ligation for the final predator: J37019 + J37025 & subsequent electroporation *Repeat ligation of J37018 ? (has not been completed & successful yet !)
Maxipreps
- Maxipreped J37022(1) (colonies a&b), J37025(3) (colonies b&c), J27025(4) (colonies b&c).
- Unable to do all 11 maxis due to lack of maxiprep supplies.
Minipreps
- Minipreped J37022(1) (colonies a,b,c,d), J37025(3) (colonies a,b,c,d), J27025(4) (colonies a,b,c,d).
Ligations
- Re-ligating J37018
- Ligating J37036 (one of the final ones...)
Culturing
- Cultured up 2ml of T9002, J37015, J37015RS, J37016 and J37020 from frozen stick
- Cultured up 100ml of aiiA from Dr.Frey in LB medium for maxiprep
- Cultured up 2ml of 4 different J37022 colonies
- Cultured up 2ml of 4 different J37025 colonies
Electroporation
- Had a bit of problem with continual blowing up, so used Kirsten's machine
- Successfully electroporated J37031 + 9G
- Unsuccessful for J37025 + J37019 (repeat tomorrow morning
