IGEM:IMPERIAL/2006/LabCalendar/2006-9-4

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==ligations==
 +
*J04500 (vector) and J37024 (insert)being ligated overnight
==LoxP PCR==
==LoxP PCR==
 +
*Was an amazing success
 +
*Jonny is a hero
==LoxP PCR fusion==
==LoxP PCR fusion==

Revision as of 12:28, 4 September 2006



TO DO:
*Testing of T9002, J37016, J37020, S01656, J37015, J37015RS
*Culture J37036 & J37018 from plate in incubator for maxi & mini on Tuesday
*Make frozen stock of J37022 & culture up for testing ! 
*Send J37024 for sequencing !!!
*Order new maxiprep kit !!!
*Need more autoclaved flasks !!!
*Need more isopropanol too!

Contents

ligations

  • J04500 (vector) and J37024 (insert)being ligated overnight

LoxP PCR

  • Was an amazing success
  • Jonny is a hero

LoxP PCR fusion

Western Blot

Maxiprep

  • Maxiprep new AiiA gene from overnight culture.
  • Not very much bacteria (hence DNA) present.

AiiA PCR

  • Using the new primers and the newly arrived AiiA gene (maxi-preped this morning), PCR carried oput to add the LVA tag and immunotag.
  • PCR annealing temperatures calculated to be 10 cyles at 51[[:Category:{{{1}}}|{{{1}}}]] and 20 cycles at 69[[:Category:{{{1}}}|{{{1}}}]]
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