IGEM:IMPERIAL/2006/LabCalendar/2006-9-4
From OpenWetWare
(Difference between revisions)
| Line 14: | Line 14: | ||
</font color> | </font color> | ||
| + | ==ligations== | ||
| + | *J04500 (vector) and J37024 (insert)being ligated overnight | ||
==LoxP PCR== | ==LoxP PCR== | ||
| + | *Was an amazing success | ||
| + | *Jonny is a hero | ||
==LoxP PCR fusion== | ==LoxP PCR fusion== | ||
Revision as of 12:28, 4 September 2006
- 2006-9-4 Events
- Day in the lab
- Project Progress
TO DO: *Testing of T9002, J37016, J37020, S01656, J37015, J37015RS *Culture J37036 & J37018 from plate in incubator for maxi & mini on Tuesday *Make frozen stock of J37022 & culture up for testing ! *Send J37024 for sequencing !!! *Order new maxiprep kit !!! *Need more autoclaved flasks !!! *Need more isopropanol too!
Contents |
ligations
- J04500 (vector) and J37024 (insert)being ligated overnight
LoxP PCR
- Was an amazing success
- Jonny is a hero
LoxP PCR fusion
Western Blot
Maxiprep
- Maxiprep new AiiA gene from overnight culture.
- Not very much bacteria (hence DNA) present.
AiiA PCR
- Using the new primers and the newly arrived AiiA gene (maxi-preped this morning), PCR carried oput to add the LVA tag and immunotag.
- PCR annealing temperatures calculated to be 10 cyles at 51[[:Category:{{{1}}}|{{{1}}}]] and 20 cycles at 69[[:Category:{{{1}}}|{{{1}}}]]
