IGEM:IMPERIAL/2006/LabCalendar/2006-9-5: Difference between revisions

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  *Make more LB & send to autoclave !!!
  *Make more LB & send to autoclave !!!
  </font color>
  </font color>
==Sequencing==
*Sent off J37015RS for sequencing (Primer = Riboswitch primer; Tm = 44{{c}}
*Sent off J37020 for sequencing (Primer = plasmid A2 reverse primer; Tm = 56{{c}}
*Sent off J37023 for sequencing (Primer = T3 and T7 primers(from Dr.Mann); Tm = 39{{c}} for both
*Sent off J37025 for sequencing (Primer = plasmid A2 forward primer; Tm = 50{{c}}

Revision as of 05:46, 5 September 2006



TO DO:
*Testing of J37016, S01656
*Finish ligation for J37022 & electroporate (has it been finished yesterday?)
*Finish ligation for Dr. Frays aiiA into the T hanging plasmid & electroporate
*Culture J37036 & J37018 from plate in incubator for maxi & mini on Wednesday
*Culture T9002 from plate in incubator for new testing & making frozen stocks
*Make more LB & send to autoclave !!!

Sequencing

  • Sent off J37015RS for sequencing (Primer = Riboswitch primer; Tm = 44[[:Category:{{{1}}}|{{{1}}}]]
  • Sent off J37020 for sequencing (Primer = plasmid A2 reverse primer; Tm = 56[[:Category:{{{1}}}|{{{1}}}]]
  • Sent off J37023 for sequencing (Primer = T3 and T7 primers(from Dr.Mann); Tm = 39[[:Category:{{{1}}}|{{{1}}}]] for both
  • Sent off J37025 for sequencing (Primer = plasmid A2 forward primer; Tm = 50[[:Category:{{{1}}}|{{{1}}}]]
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