IGEM:IMPERIAL/2006/LabCalendar/2006-9-5

• IGEM:IMPERIAL/2006/LabCalendar/2006-9-5 Events
• Day in the lab
• Project Progress

TO DO:
*Testing of J37016, S01656
*Finish ligation for J37022 & electroporate (has it been finished yesterday?)
*Finish ligation for Dr. Frays aiiA into the T hanging plasmid & electroporate
*Culture J37036 & J37018 from plate in incubator for maxi & mini on Wednesday
*Culture T9002 from plate in incubator for new testing & making frozen stocks

Western Blot

• There was no discernable difference in the proteins produced by the induced and non-induced cells
• This indicates that there is something wrong with the mechanism involving S01656 expression, possibly its promoter
• This does not however mean that the aiiA is wrong necessarily - testing of J37022 should clarify this.

LoxP PCR

• The small PCR fragment is not being amplified

Testing S01656 & J37016

• Testing was not conducted as western blot indicates that there may be something wrong with the S01656 expression mechanism.
• J37016 was being cultured for the S01656 testing. This test, too, was not finished since we are no longer carrying out testing of S01656. We have 3 good sets of data for J37016 so far.

Sequencing

Part	Primer used	Melting temp (Tm)
J37015RS	Riboswitch	44[[:Category:{{{1}}}|{{{1}}}]]
J37020	Plasmid A2 reverse primer	56[[:Category:{{{1}}}|{{{1}}}]]
J37023	T3 and T7 primers (from Dr.Mann)	39[[:Category:{{{1}}}|{{{1}}}]] for both
J37025	Plasmid A2 forward primer	50[[:Category:{{{1}}}|{{{1}}}]]