IGEM:IMPERIAL/2006/LabCalendar/2006-9-5: Difference between revisions

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  *Culture T9002 from plate in incubator for new testing & making frozen stocks
  *Culture T9002 from plate in incubator for new testing & making frozen stocks
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==Western Blot==
*There was no discernable difference in the proteins produced by the induced and non-induced cells
*This indicates that there is something wrong with the mechanism involving S01656 expression, possibly its promoter
*This does not however mean that the aiiA is wrong necessarily - testing of J37022 should clarify this.
==LoxP PCR==
* The small PCR fragment is not being amplified


==Sequencing==
==Sequencing==
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==Western Blot==
*There was no discernable difference in the proteins produced by the induced and non-induced cells
*This indicates that there is something wrong with the mechanism involving S01656 expression, possibly its promoter
*This does not however mean that the aiiA is wrong necessarily - testing of J37022 should clarify this.
==LoxP PCR==
* The small PCR fragment is not being amplified

Revision as of 07:05, 5 September 2006



TO DO:
*Testing of J37016, S01656
*Finish ligation for J37022 & electroporate (has it been finished yesterday?)
*Finish ligation for Dr. Frays aiiA into the T hanging plasmid & electroporate
*Culture J37036 & J37018 from plate in incubator for maxi & mini on Wednesday
*Culture T9002 from plate in incubator for new testing & making frozen stocks

Western Blot

  • There was no discernable difference in the proteins produced by the induced and non-induced cells
  • This indicates that there is something wrong with the mechanism involving S01656 expression, possibly its promoter
  • This does not however mean that the aiiA is wrong necessarily - testing of J37022 should clarify this.

LoxP PCR

  • The small PCR fragment is not being amplified

Sequencing

Part Primer used Melting temp (Tm)
J37015RS Riboswitch 44[[:Category:{{{1}}}|{{{1}}}]]
J37020 Plasmid A2 reverse primer 56[[:Category:{{{1}}}|{{{1}}}]]
J37023 T3 and T7 primers (from Dr.Mann) 39[[:Category:{{{1}}}|{{{1}}}]] for both
J37025 Plasmid A2 forward primer 50[[:Category:{{{1}}}|{{{1}}}]]
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