IGEM:IMPERIAL/2006/LabCalendar/2006-9-7: Difference between revisions
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<font color=red>*What about the aiiA activity from Dr Fray's bacteria ? Have we cultured it ? Can we expect aiiA expression from the plasmid ? | <font color=red>*What about the aiiA activity from Dr Fray's bacteria ? Have we cultured it ? Can we expect aiiA expression from the plasmid ? | ||
</font> | </font> | ||
<font color=blue>*JS: Also, you guys need to start sending in parts to the registry, or else we won't have anything to show for all our efforts this summer! </font> | |||
Revision as of 06:11, 7 September 2006
TO DO: *Electroporate Dr. Fray's plasmid containing aiiA into DH5a If any colonies grow until evening - culture up for testing on Friday !! *Maxi & mini of J37022, aiiA (with flag tag), aiiA *Next ligation step for new predator cell (with the new aiiA) once maxiprep is finished & electroporation on same day ! *Testing of J37015, J37022, Acylace Activity (need J37016 for two of them) *Send J37022 for sequencing *Make frozen stock of new T9002, J37036, J37022 !!! *Control digest for J37036 - Has ligation worked?
*What about the aiiA activity from Dr Fray's bacteria ? Have we cultured it ? Can we expect aiiA expression from the plasmid ?
*JS: Also, you guys need to start sending in parts to the registry, or else we won't have anything to show for all our efforts this summer!
