IGEM:IMPERIAL/2006/LabCalendar/2006-9-7: Difference between revisions
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*Control digest for J37036 - Has ligation worked? | *Control digest for J37036 - Has ligation worked? | ||
</font color> | </font color> | ||
<font color=red>*What about the aiiA activity from Dr Fray's bacteria ? Have we cultured it ? Can we expect aiiA expression from the plasmid ? | |||
<font color=red>*What about the aiiA activity from Dr Fray's bacteria ? Have we cultured it ? Can we expect aiiA expression from the plasmid ?</font> | |||
<font color=blue>*JS: Also, you guys need to start sending in parts to the registry, or else we won't have anything to show for all our efforts this summer! </font> | <font color=blue>*JS: Also, you guys need to start sending in parts to the registry, or else we won't have anything to show for all our efforts this summer! </font> | ||
==Electroporation of Dr. Fray's plasmid containing aiiA== | |||
*Was electroporated this morning into Rosetta (not into DH5a because no expression will take place in DH5a due to the promoter not working in this strain) | |||
*Might get some colonies so that it can be cultured for Western Blot tomorrow | |||
Revision as of 09:21, 7 September 2006
TO DO: *Electroporate Dr. Fray's plasmid containing aiiA into DH5a If any colonies grow until evening - culture up for testing on Friday !! *Maxi & mini of J37022, aiiA (with flag tag), aiiA *Next ligation step for new predator cell (with the new aiiA) once maxiprep is finished & electroporation on same day ! *Testing of J37015, J37022, Acylace Activity (need J37016 for two of them) *Send J37022 for sequencing *Make frozen stock of new T9002, J37036, J37022 !!! *Control digest for J37036 - Has ligation worked?
*What about the aiiA activity from Dr Fray's bacteria ? Have we cultured it ? Can we expect aiiA expression from the plasmid ?
*JS: Also, you guys need to start sending in parts to the registry, or else we won't have anything to show for all our efforts this summer!
Electroporation of Dr. Fray's plasmid containing aiiA
- Was electroporated this morning into Rosetta (not into DH5a because no expression will take place in DH5a due to the promoter not working in this strain)
- Might get some colonies so that it can be cultured for Western Blot tomorrow
