IGEM:IMPERIAL/2006/Ligation Page: Difference between revisions
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==<bbpart>J37034</bbpart> (For Prey Cell)== | ==<bbpart>J37034</bbpart> (For Prey Cell)== | ||
*C0261 (24A) + I13504 (12D) | *<bbpart>C0261</bbpart> (24A) + <bbpart>I13504</bbpart> (12D) | ||
**I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp | **I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp | ||
**C0261 (24A) (vector), cut with SpeI & PstI, expected size = 661 bp + 2079 bp (pSB1A2) = 2740 bp | **C0261 (24A) (vector), cut with SpeI & PstI, expected size = 661 bp + 2079 bp (pSB1A2) = 2740 bp | ||
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==<bbpart>J37015</bbpart> (Final Prey Cell)== | ==<bbpart>J37015</bbpart> (Final Prey Cell)== | ||
*F2620 (6B) + J37034 | *<bbpart>F2620</bbpart> (6B) + <bbpart>J37034</bbpart> | ||
**J37024 (insert), cut with XbaI & PstI, expected size = 1544 bp | **J37024 (insert), cut with XbaI & PstI, expected size = 1544 bp | ||
**F2620 (6B) (vector), cut with SpeI & PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp | **F2620 (6B) (vector), cut with SpeI & PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp | ||
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==J37015RS (Final Prey Cell with Riboswitch)== | ==J37015RS (Final Prey Cell with Riboswitch)== | ||
*F2620 + (RS + J37034) | *<bbpart>F2620</bbpart> + (RS + <bbpart>J37034</bbpart>) | ||
==<bbpart>J37016</bbpart> (Final Polycistronic Predator cell Test Construct)== | ==<bbpart>J37016</bbpart> (Final Polycistronic Predator cell Test Construct)== | ||
*J37019 + I13504 (12D) | *<bbpart>J37019</bbpart> + <bbpart>I13504</bbpart> (12D) | ||
**I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp | **I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp | ||
**J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2)= 2941 bp | **J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2)= 2941 bp | ||
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==<bbpart>J37018</bbpart>== | ==<bbpart>J37018</bbpart>== | ||
*J37031 + R0062 (9G) | *<bbpart>J37031</bbpart> + <bbpart>R0062</bbpart> (9G) | ||
**J37031 (insert), cut with EcoRI & SpeI, expected size = 999 bp | **J37031 (insert), cut with EcoRI & SpeI, expected size = 999 bp | ||
**R0062 (9G) (vector), cut with EcoRI & XbaI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp | **R0062 (9G) (vector), cut with EcoRI & XbaI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp | ||
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==<bbpart>J37033</bbpart> (RBS + LuxR)== | ==<bbpart>J37033</bbpart> (RBS + LuxR)== | ||
*B0034 (3O) +C0062 (7A) | *<bbpart>B0034</bbpart> (3O) + <bbpart>C0062</bbpart> (7A) | ||
**C0062 (7A) (insert) cut with XbaI & PstI, expected size = 756 bp | **C0062 (7A) (insert) cut with XbaI & PstI, expected size = 756 bp | ||
**B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 bp (pSB1A2) = 2091 bp | **B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 bp (pSB1A2) = 2091 bp | ||
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==<bbpart>J37019</bbpart> (For Predator Cell)== | ==<bbpart>J37019</bbpart> (For Predator Cell)== | ||
*R0062 (9G) + J37033 | *<bbpart>R0062</bbpart> (9G) + <bbpart>J37033</bbpart> | ||
**J37033 (insert) cut with XbaI & PstI, expected size = 862 bp | **J37033 (insert) cut with XbaI & PstI, expected size = 862 bp | ||
**R0062 (9G) (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp | **R0062 (9G) (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp | ||
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==<bbpart>J37020</bbpart> (Final monocistronic predator test construct)== | ==<bbpart>J37020</bbpart> (Final monocistronic predator test construct)== | ||
*J37031 + J37032 | *<bbpart>J37031</bbpart> + <bbpart>J37032</bbpart> | ||
**J37032 (insert) cut with XbaI & PstI, expected size = 938 bp | **J37032 (insert) cut with XbaI & PstI, expected size = 938 bp | ||
**J37031 (vector) cut with SpeI & PstI, expected size = 999 bp + 3189 (pSB1AK3)= 4188 bp | **J37031 (vector) cut with SpeI & PstI, expected size = 999 bp + 3189 (pSB1AK3)= 4188 bp | ||
Line 50: | Line 50: | ||
==<bbpart>J37022</bbpart> (Final AiiA test construct)== | ==<bbpart>J37022</bbpart> (Final AiiA test construct)== | ||
*J04500 (16P) + J37025 | *<bbpart>J04500</bbpart> (16P) + <bbpart>J37025</bbpart> | ||
*J37025 (insert) cut with XbaI & PstI, expected size = 973 (on pSB1A2 = 2079 bp) | *J37025 (insert) cut with XbaI & PstI, expected size = 973 (on pSB1A2 = 2079 bp) | ||
*J04500 (16P) (vector) cut with SpeI & PstI, expected size = 220 bp + 3189 (pSB1AK3) = 3409 bp | *J04500 (16P) (vector) cut with SpeI & PstI, expected size = 220 bp + 3189 (pSB1AK3) = 3409 bp | ||
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==<bbpart>J37024</bbpart> (for AiiA predator cell/test construct)== | ==<bbpart>J37024</bbpart> (for AiiA predator cell/test construct)== | ||
*J37023 + B0015 | *<bbpart>J37023</bbpart> + <bbpart>B0015</bbpart> | ||
**J37023 (insert) cut with EcoRI & SpeI, expected size = 816 bp | **J37023 (insert) cut with EcoRI & SpeI, expected size = 816 bp | ||
**B0015 (1I) (vector) cut with EcoRI & XbaI, expected size = 129 + 3189 (pSB1AK3) = 3318 bp | **B0015 (1I) (vector) cut with EcoRI & XbaI, expected size = 129 + 3189 (pSB1AK3) = 3318 bp | ||
*Miniprep sizes expected: 953 bp & 3189 bp (pSB1AK3) | *Miniprep sizes expected: 953 bp & 3189 bp (pSB1AK3) | ||
*Status: Complete | |||
==<bbpart>J37025</bbpart> (for AiiA predator cell)== | ==<bbpart>J37025</bbpart> (for AiiA predator cell)== | ||
*B0034 (3O) + J37024 | *<bbpart>B0034</bbpart> (3O) + <bbpart>J37024</bbpart> | ||
*J37024 (3O) (insert) cut with XbaI & PstI, expected size = 953 bp (on pSB1AK3 = 3189 bp) | *J37024 (3O) (insert) cut with XbaI & PstI, expected size = 953 bp (on pSB1AK3 = 3189 bp) | ||
*B0034 (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 (pSB1A2) = 2091 bp | *B0034 (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 (pSB1A2) = 2091 bp | ||
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==<bbpart>J37031</bbpart> (For monocistronic predator test construct)== | ==<bbpart>J37031</bbpart> (For monocistronic predator test construct)== | ||
*J37019 + B0015 (1I), pLuxR + RBS + LuxR + Terminator | *<bbpart>J37019</bbpart> + <bbpart>B0015</bbpart> (1I), pLuxR + RBS + LuxR + Terminator | ||
**J37019 (insert) cut with EcoRI & SpeI, expected size = 862 bp | **J37019 (insert) cut with EcoRI & SpeI, expected size = 862 bp | ||
**B0015 (vector) cut with XbaI & EcoRI, expected size = 129 bp + 3189 (pSB1AK3) = 3318 bp | **B0015 (vector) cut with XbaI & EcoRI, expected size = 129 bp + 3189 (pSB1AK3) = 3318 bp | ||
Line 106: | Line 107: | ||
==<bbpart>J37032</bbpart> (For monocistronic predator test construct)== | ==<bbpart>J37032</bbpart> (For monocistronic predator test construct)== | ||
*R0062 (9G) + I13504 (12D), pLuxR + RBS + GFP + Terminator | *<bbpart>R0062</bbpart> (9G) + <bbpart>I13504</bbpart> (12D), pLuxR + RBS + GFP + Terminator | ||
**I13504 (insert) cut with XbaI & PstI, expected size = 875 bp | **I13504 (insert) cut with XbaI & PstI, expected size = 875 bp | ||
**R0062 (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp | **R0062 (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp | ||
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==<bbpart>J37035</bbpart> (Final Monocistronic Predator Cell)== | ==<bbpart>J37035</bbpart> (Final Monocistronic Predator Cell)== | ||
*J37018 + J37025 | *<bbpart>J37018</bbpart> + <bbpart>J37025</bbpart> | ||
**J37025 (insert), cut with XbaI & PstI, expected size = 973 bp | **J37025 (insert), cut with XbaI & PstI, expected size = 973 bp | ||
**J37018 (vector), cut with SpeI & PstI, expected size = 1062 bp + 2079 bp (pSB1A2) = 3141 bp | **J37018 (vector), cut with SpeI & PstI, expected size = 1062 bp + 2079 bp (pSB1A2) = 3141 bp | ||
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==<bbpart>J37036</bbpart> (Final Polycistronic Predator Cell)== | ==<bbpart>J37036</bbpart> (Final Polycistronic Predator Cell)== | ||
*J37019 + J37025 | *<bbpart>J37019</bbpart> + <bbpart>J37025</bbpart> | ||
**J37025 (insert), cut with XbaI & PstI, expected size = 973 bp | **J37025 (insert), cut with XbaI & PstI, expected size = 973 bp | ||
**J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2) = 2941 bp | **J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2) = 2941 bp |
Revision as of 04:03, 27 August 2006
<bbpart>J37034</bbpart> (For Prey Cell)
- <bbpart>C0261</bbpart> (24A) + <bbpart>I13504</bbpart> (12D)
- I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp
- C0261 (24A) (vector), cut with SpeI & PstI, expected size = 661 bp + 2079 bp (pSB1A2) = 2740 bp
- Miniprep sizes expected: 1544 bp & 2079 bp (pSB1A2)
<bbpart>J37015</bbpart> (Final Prey Cell)
- <bbpart>F2620</bbpart> (6B) + <bbpart>J37034</bbpart>
- J37024 (insert), cut with XbaI & PstI, expected size = 1544 bp
- F2620 (6B) (vector), cut with SpeI & PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp
- Miniprep sizes expected: 2613 bp & 2079 bp (pSB1A2)
J37015RS (Final Prey Cell with Riboswitch)
- <bbpart>F2620</bbpart> + (RS + <bbpart>J37034</bbpart>)
<bbpart>J37016</bbpart> (Final Polycistronic Predator cell Test Construct)
- <bbpart>J37019</bbpart> + <bbpart>I13504</bbpart> (12D)
- I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp
- J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2)= 2941 bp
- Miniprep sizes expected: 1745 bp & 2079 bp (pSB1A2)
- Status: Completed & Sequenced
<bbpart>J37017</bbpart> (not necessary)
<bbpart>J37018</bbpart>
- <bbpart>J37031</bbpart> + <bbpart>R0062</bbpart> (9G)
- J37031 (insert), cut with EcoRI & SpeI, expected size = 999 bp
- R0062 (9G) (vector), cut with EcoRI & XbaI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
- Miniprep sizes expected: 1062 bp & 2079 bp (pSB1A2)
<bbpart>J37033</bbpart> (RBS + LuxR)
- <bbpart>B0034</bbpart> (3O) + <bbpart>C0062</bbpart> (7A)
- C0062 (7A) (insert) cut with XbaI & PstI, expected size = 756 bp
- B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 bp (pSB1A2) = 2091 bp
- Miniprep sizes expected: 799 bp & 2079 bp (pSB1A2)
- Status: Completed
<bbpart>J37019</bbpart> (For Predator Cell)
- <bbpart>R0062</bbpart> (9G) + <bbpart>J37033</bbpart>
- J37033 (insert) cut with XbaI & PstI, expected size = 862 bp
- R0062 (9G) (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
- Miniprep sizes expected: 862 bp & 2079 bp (pSB1A2)
- Status: Completed & Sequenced
<bbpart>J37020</bbpart> (Final monocistronic predator test construct)
- <bbpart>J37031</bbpart> + <bbpart>J37032</bbpart>
- J37032 (insert) cut with XbaI & PstI, expected size = 938 bp
- J37031 (vector) cut with SpeI & PstI, expected size = 999 bp + 3189 (pSB1AK3)= 4188 bp
- Miniprep sizes expected: 1945 bp & 3189 bp (pSB1AK3)
<bbpart>J37022</bbpart> (Final AiiA test construct)
- <bbpart>J04500</bbpart> (16P) + <bbpart>J37025</bbpart>
- J37025 (insert) cut with XbaI & PstI, expected size = 973 (on pSB1A2 = 2079 bp)
- J04500 (16P) (vector) cut with SpeI & PstI, expected size = 220 bp + 3189 (pSB1AK3) = 3409 bp
- Miniprep sizes expected: 1181 bp & 3189 bp (pSB1AK3)
<bbpart>J37023</bbpart> (for predator cell/test construct)
- AiiA with Immunotag
- Done by PCR
- Status: Complete
- Expected Part Length = 816 bp
- Expected Vector Length = Unknown (Hanging T tail vector)
<bbpart>J37024</bbpart> (for AiiA predator cell/test construct)
- <bbpart>J37023</bbpart> + <bbpart>B0015</bbpart>
- J37023 (insert) cut with EcoRI & SpeI, expected size = 816 bp
- B0015 (1I) (vector) cut with EcoRI & XbaI, expected size = 129 + 3189 (pSB1AK3) = 3318 bp
- Miniprep sizes expected: 953 bp & 3189 bp (pSB1AK3)
- Status: Complete
<bbpart>J37025</bbpart> (for AiiA predator cell)
- <bbpart>B0034</bbpart> (3O) + <bbpart>J37024</bbpart>
- J37024 (3O) (insert) cut with XbaI & PstI, expected size = 953 bp (on pSB1AK3 = 3189 bp)
- B0034 (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 (pSB1A2) = 2091 bp
- Miniprep sizes expected: 973 bp & 2079 bp (pSB1A2)
<bbpart>J37026</bbpart> (Cre/Lox Part Plasmid 1)
- Lox66 Part
- Part Size = 34 bp
- Has XbaI, SpeI, PstI, and EcoRI sites
- On Blunt ended hanging T tail plasmid
<bbpart>J37027</bbpart> (Final Cre/Lox Part Plasmid 1)
- To be made by PCR
- Part Size = 1127 bp
- See John Chattaway for details
<bbpart>J37028</bbpart> (Cre/Lox Part Plasmid 1)
- Lox71 Part
- Part Size = 34 bp
<bbpart>J37029</bbpart> (Cre/Lox Part Plasmid 2)
- Cre Part
- Part Size = 1032 bp
- Received, in fridge
- Chloramphenicol resistance
- Will destroy itself once we use it
<bbpart>J37030</bbpart> (not necessary)
<bbpart>J37031</bbpart> (For monocistronic predator test construct)
- <bbpart>J37019</bbpart> + <bbpart>B0015</bbpart> (1I), pLuxR + RBS + LuxR + Terminator
- J37019 (insert) cut with EcoRI & SpeI, expected size = 862 bp
- B0015 (vector) cut with XbaI & EcoRI, expected size = 129 bp + 3189 (pSB1AK3) = 3318 bp
- Miniprep sizes expected: 999 bp & 3189 bp (pSB1AK3)
- Status: Complete
<bbpart>J37032</bbpart> (For monocistronic predator test construct)
- <bbpart>R0062</bbpart> (9G) + <bbpart>I13504</bbpart> (12D), pLuxR + RBS + GFP + Terminator
- I13504 (insert) cut with XbaI & PstI, expected size = 875 bp
- R0062 (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
- Miniprep sizes expected: 938 bp & 2079 bp (pSB1A2)
- Status: Complete
<bbpart>J37035</bbpart> (Final Monocistronic Predator Cell)
- <bbpart>J37018</bbpart> + <bbpart>J37025</bbpart>
- J37025 (insert), cut with XbaI & PstI, expected size = 973 bp
- J37018 (vector), cut with SpeI & PstI, expected size = 1062 bp + 2079 bp (pSB1A2) = 3141 bp
- Miniprep sizes expected: 2043 bp & 2079 bp (pSB1A2)
<bbpart>J37036</bbpart> (Final Polycistronic Predator Cell)
- <bbpart>J37019</bbpart> + <bbpart>J37025</bbpart>
- J37025 (insert), cut with XbaI & PstI, expected size = 973 bp
- J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2) = 2941 bp
- Miniprep sizes expected: 1843 bp & 2079 bp (pSB1A2)