IGEM:IMPERIAL/2006/Ligation Page

<bbpart>J37015</bbpart> (Final Prey Cell)

• <bbpart>F2620</bbpart> (6B) + <bbpart>J37034</bbpart>
  • J37024 (insert), cut with XbaI & PstI, expected size = 1544 bp
  • F2620 (6B) (vector), cut with SpeI & PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp
• Miniprep sizes expected: 2613 bp & 2079 bp (pSB1A2)
• Status: Completed & Sequenced

J37015RS (Final Prey Cell with Riboswitch)

• <bbpart>F2620</bbpart> + (RS + <bbpart>J37034</bbpart>)
• Status: Completed (awaiting sequencing results)

<bbpart>J37016</bbpart> (Final Polycistronic Predator cell Test Construct)

• <bbpart>J37019</bbpart> + <bbpart>I13504</bbpart> (12D)
  • I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp
  • J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2)= 2941 bp
• Miniprep sizes expected: 1745 bp & 2079 bp (pSB1A2)
• Status: Completed & Sequenced

<bbpart>J37018</bbpart>

• <bbpart>J37031</bbpart> + <bbpart>R0062</bbpart> (9G)
  • J37031 (insert), cut with EcoRI & SpeI, expected size = 957 bp
  • R0062 (9G) (vector), cut with EcoRI & XbaI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
• Miniprep sizes expected: 1012 bp & 2079 bp (pSB1A2)
• Status: Incomplete

<bbpart>J37019</bbpart> (For Predator Cell)

• <bbpart>R0062</bbpart> (9G) + <bbpart>J37033</bbpart>
  • J37033 (insert) cut with XbaI & PstI, expected size = 862 bp
  • R0062 (9G) (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
• Miniprep sizes expected: 862 bp & 2079 bp (pSB1A2)
• Status: Completed & Sequenced

<bbpart>J37020</bbpart> (Final monocistronic predator test construct)

• <bbpart>J37031</bbpart> + <bbpart>J37032</bbpart>
  • J37032 (insert) cut with XbaI & PstI, expected size = 938 bp
  • J37031 (vector) cut with SpeI & PstI, expected size = 957 bp + 3189 (pSB1AK3)= 4146 bp
• Miniprep sizes expected: 1895 bp & 3189 bp (pSB1AK3)
• Status: J37020 with terminator B0015 has been completed (awaiting sequencing results) however, we are trying to religate this part using the B0014 terminator.

<bbpart>J37022</bbpart> (Final AiiA test construct)

• <bbpart>J04500</bbpart> (16P) + <bbpart>J37024</bbpart>
• J37024 (insert) cut with XbaI & PstI, expected size = 911 bp (on pSB1AK3 = 3189 bp)
• J04500 (16P) (vector) cut with SpeI & PstI, expected size = 220 bp + 3189 (pSB1AK3) = 3409 bp
• Miniprep sizes expected: 1131 bp & 3189 bp (pSB1AK3)
• Status: Incomplete

<bbpart>J37023</bbpart> (for predator cell/test construct)

• AiiA with Immunotag
• Done by PCR
• Status: Complete (awaiting sequencing results)
• Expected Part Length = 816 bp
• Expected Vector Length = Unknown (Hanging T tail vector)

<bbpart>J37024</bbpart> (for AiiA predator cell/test construct)

• <bbpart>J37023</bbpart> + <bbpart>B0014</bbpart>
  • J37023 (insert) cut with EcoRI & SpeI, expected size = 816 bp
  • B0014 (1G) (vector) cut with EcoRI & XbaI, expected size = 95 bp + 3189 bp (pSB1AK3) = 3284 bp
• Miniprep sizes expected: 911 bp & 3189 bp (pSB1AK3)
• Status: Complete (not sequenced)

<bbpart>J37025</bbpart> (for AiiA predator cell)

• <bbpart>B0034</bbpart> (3O) + <bbpart>J37024</bbpart>
• J37024 (insert) cut with XbaI & PstI, expected size = 911 bp (on pSB1AK3 = 3189 bp)
• B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 (pSB1A2) = 2091 bp
• Miniprep sizes expected: 923 bp & 2079 bp (pSB1A2)
• Status: Incomplete

<bbpart>J37026</bbpart> (Cre/Lox Part Plasmid 1)

• Lox66 Part
• Part Size = 34 bp
• Has XbaI, SpeI, PstI, and EcoRI sites
• On Blunt ended hanging T tail plasmid

<bbpart>J37027</bbpart> (Final Cre/Lox Part Plasmid 1)

• To be made by PCR
• Part Size = 1127 bp
• See John Chattaway for details

<bbpart>J37028</bbpart> (Cre/Lox Part Plasmid 1)

• Lox71 Part
• Part Size = 34 bp

<bbpart>J37029</bbpart> (Cre/Lox Part Plasmid 2)

• Cre Part
• Part Size = 1032 bp
• Received, in fridge
• Chloramphenicol resistance
• Will destroy itself once we use it

<bbpart>J37031</bbpart> (For monocistronic predator test construct)

• <bbpart>J37019</bbpart> + <bbpart>B0014</bbpart> (1I), pLuxR + RBS + LuxR + Terminator
  • J37019 (insert) cut with EcoRI & SpeI, expected size = 862 bp
  • B0015 (vector) cut with XbaI & EcoRI, expected size = 95 bp + 3189 bp (pSB1AK3) = 3284 bp
• Miniprep sizes expected: 957 bp & 3189 bp (pSB1AK3)
• Status: Complete (not sequenced)

<bbpart>J37032</bbpart> (For monocistronic predator test construct)

• <bbpart>R0062</bbpart> (9G) + <bbpart>I13504</bbpart> (12D), pLuxR + RBS + GFP + Terminator
  • I13504 (insert) cut with XbaI & PstI, expected size = 875 bp
  • R0062 (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
• Miniprep sizes expected: 938 bp & 2079 bp (pSB1A2)
• Status: Complete

<bbpart>J37033</bbpart> (RBS + LuxR)

• <bbpart>B0034</bbpart> (3O) + <bbpart>C0062</bbpart> (7A)
  • C0062 (7A) (insert) cut with XbaI & PstI, expected size = 756 bp
  • B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 bp (pSB1A2) = 2091 bp
• Miniprep sizes expected: 799 bp & 2079 bp (pSB1A2)
• Status: Completed

<bbpart>J37034</bbpart> (For Prey Cell)

• <bbpart>C0261</bbpart> (24A) + <bbpart>I13504</bbpart> (12D)
  • I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp
  • C0261 (24A) (vector), cut with SpeI & PstI, expected size = 661 bp + 2079 bp (pSB1A2) = 2740 bp
• Miniprep sizes expected: 1544 bp & 2079 bp (pSB1A2)
• Status: Completed

<bbpart>J37035</bbpart> (Final Monocistronic Predator Cell)

• <bbpart>J37018</bbpart> + <bbpart>J37025</bbpart>
  • J37025 (insert), cut with XbaI & PstI, expected size = 923 bp
  • J37018 (vector), cut with SpeI & PstI, expected size = 1012 bp + 2079 bp (pSB1A2) = 3141 bp
• Miniprep sizes expected: 1953 bp & 2079 bp (pSB1A2)
• Status: Incomplete

<bbpart>J37036</bbpart> (Final Polycistronic Predator Cell)

• <bbpart>J37019</bbpart> + <bbpart>J37025</bbpart>
  • J37025 (insert), cut with XbaI & PstI, expected size = 923 bp
  • J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2) = 2941 bp
• Miniprep sizes expected: 1785 bp & 2079 bp (pSB1A2)
• Status: Incomplete

Ligation Chart

Ligation Chart (ppt.)

AiiA ligation chart

New Ligations

Ligation Chart (Updated: 14/08)