IGEM:IMPERIAL/2006/Ligation Page

J37016 (Final Polycistronic Predator cell Test Construct)

• J37019 + I13504 (
  • I13504 () (insert), cut with XbaI & PstI, expected size = 875 bp
  • J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2)= 2941 bp
• Miniprep sizes expected: 1745 bp & 2079 bp (pSB1A2)
• Status: Completed & Sequenced

J37033 (RBS + LuxR)

• B0034 (3O) +C0062 (7A)
  • C0062 (7A) (insert) cut with XbaI & PstI, expected size = 756 bp
  • B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 bp (pSB1A2) = 2091 bp
• Miniprep sizes expected: 799 bp & 2079 bp (pSB1A2)
• Status: Completed

J37019 (For Predator Cell)

• R0062 (9G) + J37033
  • J37033 (insert) cut with XbaI & PstI, expected size = 862 bp
  • R0062 (9G) (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
• Miniprep sizes expected: 862 bp & 2079 bp (pSB1A2)
• Status: Completed & Sequenced

J37020 (Final monocistronic predator test construct)

• J37031 + J37032
  • J37032 (insert) cut with XbaI & PstI, expected size = 938 bp
  • J37031 (vector) cut with SpeI & PstI, expected size = 999 bp + 3189 (pSB1AK3)= 4188 bp
• Miniprep sizes expected: 1945 bp & 3189 bp (pSB1AK3)

J37022 (Final AiiA test construct)

• J04500 (16P) + J37025
• J37025 (insert) cut with XbaI & PstI, expected size = 973 (on pSB1A2 = 2079 bp)
• J04500 (16P) (vector) cut with SpeI & PstI, expected size = 220 bp + 3189 (pSB1AK3) = 3409 bp
• Miniprep sizes expected: 1181 bp & 3189 bp (pSB1AK3)

J37023 (for predator cell/test construct)

• AiiA with Immunotag
• Done by PCR
• Status: Complete
• Expected Part Length = 816 bp
• Expected Vector Length = Unknown (Hanging T tail vector)

J37024 (for AiiA predator cell/test construct)

• J37023 + B0015
  • J37023 (insert) cut with EcoRI & SpeI, expected size = 816 bp
  • B0015 (1I) (vector) cut with EcoRI & XbaI, expected size = 129 + 3189 (pSB1AK3) = 3318 bp
• Miniprep sizes expected: 953 bp & 3189 bp (pSB1AK3)

J37025 (for AiiA predator cell)

• B0034 (3O) + J37024
• J37024 (3O) (insert) cut with XbaI & PstI, expected size = 953 bp (on pSB1AK3 = 3189 bp)
• B0034 (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 (pSB1A2) = 2091 bp
• Miniprep sizes expected: 973 bp & 2079 bp (pSB1A2)

J37026 (Cre/Lox Part Plasmid 1)

• Lox66 Part
• Part Size = 34 bp
• Has XbaI, SpeI, PstI, and EcoRI sites
• On Blunt ended hanging T tail plasmid

J37027 (Final Cre/Lox Part Plasmid 1)

• To be made by PCR
• Part Size = 1127 bp
• See John Chattaway for details

J37028 (Cre/Lox Part Plasmid 1)

• Lox71 Part
• Part Size = 34 bp

J37029 (Cre/Lox Part Plasmid 2)

• Cre Part
• Part Size = 1032 bp
• Received, in fridge
• Chloramphenicol resistance
• Will destroy itself once we use it

J37031 (For monocistronic predator test construct)

• J37019 + B0015 (1I), pLuxR + RBS + LuxR + Terminator
  • J37019 (insert) cut with EcoRI & SpeI, expected size = 862 bp
  • B0015 (vector) cut with XbaI & EcoRI, expected size = 129 bp + 3189 (pSB1AK3) = 3318 bp
• Miniprep sizes expected: 999 bp & 3189 bp (pSB1AK3)
• Status: Complete

J37032 (For monocistronic predator test construct)

• R0062 (9G) + I13504 (12D), pLuxR + RBS + GFP + Terminator
  • I13504 (insert) cut with XbaI & PstI, expected size = 875 bp
  • R0062 (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
• Miniprep sizes expected: 938 bp & 2079 bp (pSB1A2)
• Status: Complete