IGEM:IMPERIAL/2006/ProjectCalendar/2006-9-4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
mNo edit summary |
||
| Line 47: | Line 47: | ||
**Have you already done some testing ? Is there a protocol ? | **Have you already done some testing ? Is there a protocol ? | ||
**How promising is it ? Future work ? | **How promising is it ? Future work ? | ||
*Other issues on testing ? | |||
==Modelling== | ==Modelling== | ||
| Line 52: | Line 54: | ||
*Do we have simulations using realistic values ? | *Do we have simulations using realistic values ? | ||
*How likely our final construct is to work according to simulations ? | *How likely our final construct is to work according to simulations ? | ||
*Other issues on modelling ? | |||
==Doc & Presentation== | ==Doc & Presentation== | ||
*Is the wiki up to date according to the work done during the last two weeks ? | *Is the wiki up to date according to the work done during the last two weeks ? | ||
*Have we started to work on the presentation of the project for the Jamboree ? | *Have we started to work on the presentation of the project for the Jamboree ? | ||
*Other issues on documentation ? | |||
Revision as of 04:08, 4 September 2006
Progress Report
Assemly
- Define the status of each test construct (under construction, sequenced, validated, faulty, ready for testing, tested, characterized) -multiple choice possible-
- Prey cell:
- Prey cell with riboswitch:
- Prey cell with Cre:
- Predator sensing part (monocistronic):
- Predator sensing part (polycistronic):
- aiiA test construct:
- Full Predator construct:
- others ???
- What is the current priority in terms of assembly process ? Why ?
- Other issues ?
Testing
- Define the status of each testing protocol (to be written, to be validated, validated, data collected, data analysed, faulty protocol) -multiple choices possible-
- Prey cell:
- Prey cell with riboswitch:
- Prey cell with Cre:
- Predator sensing part (mono):
- Predator sensing part (poly):
- aiiA test construct:
- Full system:
- aiiA from MIT:
- What lead us to think that the MIT aiiA part is faulty ?
- Have we tried to be in touch with other iGEM teams using it ?
- We have received a new aiiA gene. Can we test it before trying to insert it into our construct ?
- Chemostat
- What is the medium to be used in the chemostat ? At which OD do you plan to operate ?
- Which parts do we plan to test in Chemostat ? Protocols ? Do we know how to get the parameters of the part from the experiment ?
- Have we done the growth curves of the parts we want to test in the chemostat ?
- Do we have a final strategy to monitor the ratio between our two populations ?
- Do we understand how the chemostat environment is going to modify our modelling approach ?
- Biosensor
- What is the name of the enzyme we are using ? reference ?
- Have you already done some testing ? Is there a protocol ?
- How promising is it ? Future work ?
- Other issues on testing ?
Modelling
- What is the status on the theoretical study of the system ?
- Do we have simulations using realistic values ?
- How likely our final construct is to work according to simulations ?
- Other issues on modelling ?
Doc & Presentation
- Is the wiki up to date according to the work done during the last two weeks ?
- Have we started to work on the presentation of the project for the Jamboree ?
- Other issues on documentation ?
