IGEM:IMPERIAL/2006/Protocols: Difference between revisions
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*[[IGEM:IMPERIAL/2006/Protocols/fusion PCR|Fusion PCR Protocol]] | *[[IGEM:IMPERIAL/2006/Protocols/fusion PCR|Fusion PCR Protocol]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/western_blot|Western Blot Protocol]] | *[[IGEM:IMPERIAL/2006/Protocols/western_blot|Western Blot Protocol]] | ||
*[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]] | |||
*[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) | *[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) | ||
*[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)] | *[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)] | ||
Revision as of 11:06, 29 October 2006
Protocols
- LB Amp Plate
- Transformations
- Competent Cells
- Bradford Assay
- 20 μL Digestion
- 20 μL Ligation
- 30 μL Ligation
- Miniprep of Culture
- 50x TAE Solution
- Agarose Gel
- Measuring DNA Concentration
- Fusion PCR Protocol
- Western Blot Protocol
- 1 kb Plus DNA Ladder
- Freezing cells (Protocol from OWW: Making a long term stock of bacteria)
