IGEM:IMPERIAL/2006/Protocols

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<ul>
<ul>
<li>[[IGEM:IMPERIAL/2006/Protocols |General Protocols]]</li>
<li>[[IGEM:IMPERIAL/2006/Protocols |General Protocols]]</li>
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<li>[[IGEM:IMPERIAL/2006/Protocols/Testing |Testing Protocol]]</li>
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<li>[[IGEM:IMPERIAL/2006/Protocols/Testing |Testing Protocols]]</li>
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</ul>
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</div>
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<br style="clear:both">
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===[[IGEM:IMPERIAL/2006|Main Page]]===
 
==Protocols==
==Protocols==
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*[[IGEM:IMPERIAL/Protocols/bacterial_culture|Bacterial Culture]]
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*[[IGEM:IMPERIAL/2006/Protocols/LB_Amp_plate|LB Amp Plate]]
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*[[IGEM:IMPERIAL/Protocols/LB_Amp_plate|LB Amp Plate]]
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*[[IGEM:IMPERIAL/2006/Protocols/transformation|Transformations]]
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*[[IGEM:IMPERIAL/Protocols/transformation|Transformations]]
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*[[IGEM:IMPERIAL/2006/Protocols/competent_cells_DH5a|Competent Cells]]
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*[[IGEM:IMPERIAL/Protocols/competent_cells_DH5a|Competent Cells]]
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*[[IGEM:IMPERIAL/2006/Protocols/bradford_assay|Bradford Assay]]
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*[[IGEM:IMPERIAL/Protocols/bradford_assay|Bradford Assay]]
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*[[IGEM:IMPERIAL/2006/Protocols/20ul_digestion|20 {{uL}} Digestion]]
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*[[IGEM:IMPERIAL/Protocols/20ul_digestion|20 uL Digestion]]
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*[[IGEM:IMPERIAL/2006/Protocols/20ul_ligation|20 {{uL}} Ligation]]
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*[[IGEM:IMPERIAL/Protocols/20ul_ligation|20 uL Ligation]]
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*[[IGEM:IMPERIAL/2006/Protocols/30ul_ligation|30 {{uL}} Ligation]]
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*[[IGEM:IMPERIAL/Protocols/30ul_ligation|30 uL Ligation]]
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*[[IGEM:IMPERIAL/2006/Protocols/mini_prep_of_culture|Miniprep of Culture]]
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*[[IGEM:IMPERIAL/Protocols/mini_prep_of_culture|Miniprep of Culture]]
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*[[IGEM:IMPERIAL/2006/Protocols/50X_TAE|50x TAE Solution]]
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*[[IGEM:IMPERIAL/Protocols/50X_TAE|50x TAE Solution]]
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*[[IGEM:IMPERIAL/2006/Protocols/agarose_gel|Agarose Gel]]
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*[[IGEM:IMPERIAL/Protocols/agarose_gel|Agarose Gel]]
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*[[IGEM:IMPERIAL/2006/Protocols/measuring_dna_concentration|Measuring DNA Concentration]]
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*[[IGEM:IMPERIAL/Protocols/measuring_dna_concentration|Measuring DNA Concentration]]
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*[[IGEM:IMPERIAL/2006/Protocols/fusion PCR|Fusion PCR Protocol]]
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*[[IGEM:IMPERIAL/Protocols/fusion PCR|Fusion PCR protocol]]
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*[[IGEM:IMPERIAL/2006/Protocols/western_blot|Western Blot Protocol]]
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*[[IGEM:IMPERIAL/Protocols/western_blot|Western Blot Protocol]]
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*[[IGEM:IMPERIAL/Protocols/Protocal_for_aiia_PCR|Protocal for aiia PCR]]
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*[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]]
*[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]]
*[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria)
*[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria)
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*[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)]
*[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)]
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==Part Testing Protocols==
 
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*[[IGEM:IMPERIAL/2006/Protocols/T9002|T9002/J37016/J37020 Testing Protocol]]
 
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*[[IGEM:IMPERIAL/2006/Protocols/J37015|J37015 Testing Protocols]]
 
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*[[IGEM:IMPERIAL/Protocols/J37015-LuxI|J37015-LuxI Testing Protocol]] <font color=red> PRELIMINARY VERSION </font>
 
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*[[IGEM:IMPERIAL/Protocols/I13207|I13207 Testing Protocol]] <font color=red> UNFINISHED</font> (This part is no longer necessary)
 
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*[[IGEM:IMPERIAL/Protocols/J37022|J37022 Testing Protocol]]
 
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*[[IGEM:IMPERIAL/Protocols/S01656|S01656 Testing Protocol]]
 
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*[[IGEM:IMPERIAL/2006/Protocols/S01656rev|S01656 revised Testing Protocol]]
 
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*[[IGEM:IMPERIAL/Protocols/S01656new|New S0165 testing protocol]]
 
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===[[IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-17 | >>>Current issues on testing protocols ...]]===
 
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==Biosensor Testing Protocol==
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*[[IGEM:IMPERIAL/2006/Protocols/Biosensortest|Biosensor Testing: Calibration & Characterization]]
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*[[IGEM:IMPERIAL/2006/Protocols/BiosensorEnzymeAct|Biosensor Testing: Enzyme Activity]]
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