IGEM:IMPERIAL/2006/Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 15: | Line 15: | ||
*[[IGEM:IMPERIAL/2006/Protocols/Bacterial_Culture|Bacterial Culture]] | *[[IGEM:IMPERIAL/2006/Protocols/Bacterial_Culture|Bacterial Culture]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/LB_Amp_plate|LB Amp Plate]] | *[[IGEM:IMPERIAL/2006/Protocols/LB_Amp_plate|LB Amp Plate]] | ||
*[[IGEM:IMPERIAL/Protocols/transformation|Transformations]] | *[[IGEM:IMPERIAL/2006/Protocols/transformation|Transformations]] | ||
*[[IGEM:IMPERIAL/Protocols/competent_cells_DH5a|Competent Cells]] | *[[IGEM:IMPERIAL/2006/Protocols/competent_cells_DH5a|Competent Cells]] | ||
*[[IGEM:IMPERIAL/Protocols/bradford_assay|Bradford Assay]] | *[[IGEM:IMPERIAL/2006/Protocols/bradford_assay|Bradford Assay]] | ||
*[[IGEM:IMPERIAL/Protocols/20ul_digestion|20 uL Digestion]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_digestion|20 uL Digestion]] | ||
*[[IGEM:IMPERIAL/Protocols/20ul_ligation|20 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_ligation|20 uL Ligation]] | ||
*[[IGEM:IMPERIAL/Protocols/30ul_ligation|30 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/30ul_ligation|30 uL Ligation]] | ||
*[[IGEM:IMPERIAL/Protocols/mini_prep_of_culture|Miniprep of Culture]] | *[[IGEM:IMPERIAL/2006/Protocols/mini_prep_of_culture|Miniprep of Culture]] | ||
*[[IGEM:IMPERIAL/Protocols/50X_TAE|50x TAE Solution]] | *[[IGEM:IMPERIAL/2006/Protocols/50X_TAE|50x TAE Solution]] | ||
*[[IGEM:IMPERIAL/Protocols/agarose_gel|Agarose Gel]] | *[[IGEM:IMPERIAL/2006/Protocols/agarose_gel|Agarose Gel]] | ||
*[[IGEM:IMPERIAL/Protocols/measuring_dna_concentration|Measuring DNA Concentration]] | *[[IGEM:IMPERIAL/2006/Protocols/measuring_dna_concentration|Measuring DNA Concentration]] | ||
*[[IGEM:IMPERIAL/Protocols/fusion PCR|Fusion PCR protocol]] | *[[IGEM:IMPERIAL/2006/Protocols/fusion PCR|Fusion PCR protocol]] | ||
*[[IGEM:IMPERIAL/Protocols/western_blot|Western Blot Protocol]] | *[[IGEM:IMPERIAL/2006/Protocols/western_blot|Western Blot Protocol]] | ||
*[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]] | *[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]] | ||
*[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) | *[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) |
Revision as of 05:15, 28 October 2006
Main Page
Protocols
- Bacterial Culture
- LB Amp Plate
- Transformations
- Competent Cells
- Bradford Assay
- 20 uL Digestion
- 20 uL Ligation
- 30 uL Ligation
- Miniprep of Culture
- 50x TAE Solution
- Agarose Gel
- Measuring DNA Concentration
- Fusion PCR protocol
- Western Blot Protocol
- 1 kb Plus DNA Ladder
- Freezing cells (Protocol from OWW: Making a long term stock of bacteria)