IGEM:IMPERIAL/2006/Protocols/20ul digestion

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20μL digestion

  • Place the following in an Eppendorf:
    • 5~8μg DNA (inserts, if vector, max of 0.5μg)
    • 2μL 10x buffer
    • 2μL Enzyme – add last (must be kept cold at all times) - (should be less than 10% of the total volume otherwise glycerol will inhibit the enzyme activity)
    • Make it up to 20μL with MiliQ

  • 1hr at 37[[:Category:{{{1}}}|{{{1}}}]].
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