IGEM:IMPERIAL/2006/Protocols/30ul ligation: Difference between revisions
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30 uL | == 30{{uL}} Ligation == | ||
*25 uL DNA | |||
*3 uL Buffer | 30{{uL}} ligations are to be used for cutting parts out for ligation. Be sure to use a large gel with NEW TAE in the tank. | ||
*1 uL of each enzyme | |||
*25{{uL}} DNA | |||
*3{{uL}} Buffer | |||
*1{{uL}} of each enzyme | |||
Latest revision as of 06:23, 28 October 2006
30μL Ligation
30μL ligations are to be used for cutting parts out for ligation. Be sure to use a large gel with NEW TAE in the tank.
- 25μL DNA
- 3μL Buffer
- 1μL of each enzyme
