IGEM:IMPERIAL/2006/Protocols/30ul ligation

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< IGEM:IMPERIAL | 2006 | Protocols
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30μL Ligation

30μL ligations are to be used for cutting parts out for ligation. Be sure to use a large gel with NEW TAE in the tank.

  • 25μL DNA
  • 3μL Buffer
  • 1μL of each enzyme
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