IGEM:IMPERIAL/2006/Protocols/30ul ligation
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30μL Ligation
30μL ligations are to be used for cutting parts out for ligation. Be sure to use a large gel with NEW TAE in the tank.
- 25μL DNA
- 3μL Buffer
- 1μL of each enzyme
30μL ligations are to be used for cutting parts out for ligation. Be sure to use a large gel with NEW TAE in the tank.