IGEM:IMPERIAL/2006/Protocols/bradford assay: Difference between revisions
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1) 1 in 5 dilution of Bradford assay solution | 1) 1 in 5 dilution of Bradford assay solution | ||
*4ml in 16ml of dH2O | *4ml in 16ml of dH2O | ||
* | *Small stock in the fridge, main stock in the cold cabinet) | ||
*(light sensitive) | *(light sensitive) | ||
2) Use 1mg/ml BSA as a standard protein | 2) Use 1mg/ml BSA as a standard protein | ||
Latest revision as of 06:17, 28 October 2006
Bradford Assay
1) 1 in 5 dilution of Bradford assay solution
- 4ml in 16ml of dH2O
- Small stock in the fridge, main stock in the cold cabinet)
- (light sensitive)
2) Use 1mg/ml BSA as a standard protein
- Dilute different amount of BSA (0.1-1μg)
3) Place 200μL of diluted protein into the well of 96 well plate.
4) Make different dilution of your sample protein and then measure absorbance at 595nm, then calculate concentration using BSA standard curve.
