IGEM:IMPERIAL/2006/Protocols/western blot

• Grow overnight in a 5 mil culture
• Make a fresh day culture 400ul of overnight culture + 4.6 ml of fresh medium (LB)
• Grow fresh culture for 1.5hrs
• Remove 1ml This will be your controll
• Add IPTG to the remaining 4ml Final conc should be 0.5-1 milimolar So add 4ul of 1 molar IPTG
• Grow for 2-3 hrs
• Centrafuge down
• Go to Keran's Lab (on the same floor as kirstin)
• Add Lysis Buffers
• Run on gel

You should get this

Source http://www.cdc.gov/ncidod/eid/vol7no1/hussainG1.htm