IGEM:IMPERIAL/2006/Results: Difference between revisions

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**JS: These results look somewhat promising
**JS: These results look somewhat promising
*[http://openwetware.org/images/a/ac/020906_J37016_processed.xls Data for J37016]
*[http://openwetware.org/images/a/ac/020906_J37016_processed.xls Data for J37016]
**JS: These results look somewhat promising
**JS: <s>These results look somewhat promising</s> Having looked at this set of data, there seems to be something wrong, as the OD of the cultures is only around 0.1.  I don't think this is reasonable considering we left the plate in the shaker for at least 4 hours, and previous experimental data would suggest an OD of approximately 0.5 after 4 hours.  I think we might have to discard this data.  Any suggestions?
*[http://openwetware.org/images/4/48/020906_J37020_processed.xls Data for J37020]
*[http://openwetware.org/images/4/48/020906_J37020_processed.xls Data for J37020]
**JS: I have to say...the results from this testing of J37020 isn't very promising
**JS: I have to say...the results from this testing of J37020 isn't very promising


*<font color=red>JS: I will try to compile all of the J37016 data within the next few days.  As I am busy with classes, I won't be able to guarantee anything, but I'll try my best.  Also, MIT uses a linux system, which is great, but unfortunately, doesn't have any Microsoft software on it, and I am not too familiar with OpenOffice, so I have to bring in my laptop one day to download all of the data :(...However, I went to a talk by Drew Endy today and when he was showing a powerpoint slide of the iGEM wiki, I was very surprised to see Imperial College's picture on the front!  At least I recognised all the people there.  He did give a great talk which perhaps would have been better if we were all given it at the beginning of the project (since this talk was given mainly to 1st and 2nd year students who are interested in their Biological Engineering major here at MIT).  None the less, it was great seeing you guys again, albeit in the most awkard place :)!
*<font color=red>JS: I will try to compile all of the J37016 data within the next few days.  As I am busy with classes, I won't be able to guarantee anything, but I'll try my best.  Also, MIT uses a linux system, which is great, but unfortunately, doesn't have any Microsoft software on it, and I am not too familiar with OpenOffice, so I have to bring in my laptop one day to download all of the data :(...However, I went to a talk by Drew Endy today and when he was showing a powerpoint slide of the iGEM wiki, I was very surprised to see Imperial College's picture on the front!  At least I recognised all the people there.  He did give a great talk which perhaps would have been better if we were all given it at the beginning of the project (since this talk was given mainly to 1st and 2nd year students who are interested in their Biological Engineering major here at MIT).  None the less, it was great seeing you guys again, albeit in the most awkard place :)!

Revision as of 06:31, 7 September 2006

Results

22/08/06

  • Data for plate 1 and plate 2 of T9002 testing (96-well plate was left in shaker overnight).

23/08/06


24/08/06

25/08/06

26/08/06

  • The wrong AHL was used so we're discounting all data gathered on this day.
  • All cultures for future testing are to be grown up from the freezer stock.

27/08/06

  • The results of the T9002 assay are not useable...carry on testing.
  • J37016 results look useable.
  • J37020 testing done with wrong culture of J37020.

28/08/06

  • The results of the T9002 assay are not useable...carry on testing.
  • J37016 results look un-useable.
  • J37020 testing done with wrong culture of J37020.

29/08/06

  • No data collected today

30/08/06

31/08/06

01/09/06

02/09/06

  • Data for T9002
    • JS: These results look somewhat promising
  • Data for J37016
    • JS: These results look somewhat promising Having looked at this set of data, there seems to be something wrong, as the OD of the cultures is only around 0.1. I don't think this is reasonable considering we left the plate in the shaker for at least 4 hours, and previous experimental data would suggest an OD of approximately 0.5 after 4 hours. I think we might have to discard this data. Any suggestions?
  • Data for J37020
    • JS: I have to say...the results from this testing of J37020 isn't very promising
  • JS: I will try to compile all of the J37016 data within the next few days. As I am busy with classes, I won't be able to guarantee anything, but I'll try my best. Also, MIT uses a linux system, which is great, but unfortunately, doesn't have any Microsoft software on it, and I am not too familiar with OpenOffice, so I have to bring in my laptop one day to download all of the data :(...However, I went to a talk by Drew Endy today and when he was showing a powerpoint slide of the iGEM wiki, I was very surprised to see Imperial College's picture on the front! At least I recognised all the people there. He did give a great talk which perhaps would have been better if we were all given it at the beginning of the project (since this talk was given mainly to 1st and 2nd year students who are interested in their Biological Engineering major here at MIT). None the less, it was great seeing you guys again, albeit in the most awkard place :)!