IGEM:IMPERIAL/2006/Results: Difference between revisions

Revision as of 06:35, 7 September 2006

Data for plate 1 and plate 2 of T9002 testing (96-well plate was left in shaker overnight).

Data for T9002
Data for J37016
- JS: Having analysed this set of data, it looks like we can use it. As from the 31/8 data, we get a response about 1 to 10 nM and a peak around 100 nM. It is curious to note that there is a decrease in fluorescence as we further increase AHL concentration past the 100 nM mark, suggesting that there might be a possible problem with the AHL concentrations. Also, we are obtaining peak fluorescences of 2700 units which is consistent with 31/8 data.
Data for J37020

Data for T9002
- JS: These results look somewhat promising
Data for J37016
- JS: ~~These results look somewhat promising~~ Having looked at this set of data, there seems to be something wrong, as the OD of the cultures is only around 0.1. I don't think this is reasonable considering we left the plate in the shaker for at least 4 hours, and previous experimental data would suggest an OD of approximately 0.5 after 4 hours. I think we might have to discard this data. Any suggestions?
Data for J37020
- JS: I have to say...the results from this testing of J37020 isn't very promising

JS: I will try to compile all of the J37016 data within the next few days. As I am busy with classes, I won't be able to guarantee anything, but I'll try my best. Also, MIT uses a linux system, which is great, but unfortunately, doesn't have any Microsoft software on it, and I am not too familiar with OpenOffice, so I have to bring in my laptop one day to download all of the data :(...However, I went to a talk by Drew Endy today and when he was showing a powerpoint slide of the iGEM wiki, I was very surprised to see Imperial College's picture on the front! At least I recognised all the people there. He did give a great talk which perhaps would have been better if we were all given it at the beginning of the project (since this talk was given mainly to 1st and 2nd year students who are interested in their Biological Engineering major here at MIT). None the less, it was great seeing you guys again, albeit in the most awkard place :)!

@@ Line 71: / Line 71: @@
 *[http://openwetware.org/images/1/1f/010906_T9002_processed.xls Data for T9002]
 *[http://openwetware.org/images/f/f4/010906_J37016_processed.xls Data for J37016]
+**JS: Having analysed this set of data, it looks like we can use it.  As from the 31/8 data, we get a response about 1 to 10 nM and a peak around 100 nM.  It is curious to note that there is a decrease in fluorescence as we further increase AHL concentration past the 100 nM mark, suggesting that there might be a possible problem with the AHL concentrations.  Also, we are obtaining peak fluorescences of 2700 units which is consistent with 31/8 data.
 *[http://openwetware.org/images/6/6a/010906_J37020_processed.xls Data for J37020]