IGEM:IMPERIAL/2006/Results/ABS490 Calibration: Difference between revisions
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==Aim== | ===Aim=== | ||
The aim of this experiment was to make a calibration curve to be able to convert Abs490, which is measured by the plate reader, into OD600. | The aim of this experiment was to make a calibration curve to be able to convert Abs490, which is measured by the plate reader, into OD600. | ||
==Method== | ===Method=== | ||
*A dilution series of different OD600s was created using the in-lab spectrophotometer and overnight culture of cells. | *A dilution series of different OD600s was created using the in-lab spectrophotometer and overnight culture of cells. | ||
| Line 10: | Line 10: | ||
*The cells and the dilutions were kept on ice to prevent any increase in OD | *The cells and the dilutions were kept on ice to prevent any increase in OD | ||
==Results== | ===Results=== | ||
*Data File: | *Data File: | ||
**[http://openwetware.org/images/4/49/Abs490od600calib.xls Abs490 -> OD600 Calibration Data] | **[http://openwetware.org/images/4/49/Abs490od600calib.xls Abs490 -> OD600 Calibration Data] | ||
Revision as of 13:03, 19 August 2006
Aim
The aim of this experiment was to make a calibration curve to be able to convert Abs490, which is measured by the plate reader, into OD600.
Method
- A dilution series of different OD600s was created using the in-lab spectrophotometer and overnight culture of cells.
- These dilutions were also assessed via the spectrophotometer to make sure they were near their targetted OD.
- 3 x 200μL of each dilution was pipetted into a 96 well plate and then Abs490 was read by the Victor3 plate-reader
- The cells and the dilutions were kept on ice to prevent any increase in OD
Results
- Data File:
