IGEM:IMPERIAL/2006/The Talk Page: Difference between revisions
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==Purpose of this Page== | ==Purpose of this Page== | ||
Tom is away revising for exams and John is going away to the US. This page is a place where questions are raised and answered and a place to keep everyone aware of what is going on. | Tom is away revising for exams and John is going away to the US. This page is a place where questions are raised and answered and a place to keep everyone aware of what is going on. | ||
==[[IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21|Monitoring Cell Populations]]== | |||
'''[[User:TomH|Tom]] 07:35, 21 August 2006 (EDT)''': Hi everyone, just been reading through [[IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21|this]]. Big drawback of the 3 antibiotic way of monitoring cell population that you've identified is the extra ligation steps. What extra ligations steps are involved? If you just mean grafting your parts into a plasmid with appropriate antibiotic markers, can't you just co-transform a seperate plasmid with the marker on, thus saving a ligation step? I'm pretty sure those kind of plasmids would be easy to get hold of as they are the basis of a lot of gene cloning. | |||
Revision as of 04:35, 21 August 2006
Purpose of this Page
Tom is away revising for exams and John is going away to the US. This page is a place where questions are raised and answered and a place to keep everyone aware of what is going on.
Monitoring Cell Populations
Tom 07:35, 21 August 2006 (EDT): Hi everyone, just been reading through this. Big drawback of the 3 antibiotic way of monitoring cell population that you've identified is the extra ligation steps. What extra ligations steps are involved? If you just mean grafting your parts into a plasmid with appropriate antibiotic markers, can't you just co-transform a seperate plasmid with the marker on, thus saving a ligation step? I'm pretty sure those kind of plasmids would be easy to get hold of as they are the basis of a lot of gene cloning.
