IGEM:IMPERIAL/2006/The Talk Page: Difference between revisions

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==Measuring LuxI==
==Measuring LuxI==


*'''[[User:Farah|Farah]] 12:22, 21 August 2006 (EDT)''': What are we doing about luxI in J37015? Are we going ahead with measuring the GFP levels in that part? Tom mentioned talking to Vincent about it...I can model the luxI but from the assumptions we've made for our current model for J37016 we ought to get a similar curve as for the AHL levels. To Tom: I remember you saying you've talked to Vincent about the luxI - what was the conclusion?
*'''[[User:Farah|Farah]] 12:22, 21 August 2006 (EDT)''': What are we doing about luxI in J37015? Are we going ahead with measuring the GFP levels in that part? Tom mentioned talking to Vincent about it...I can model the luxI but from the assumptions we've made for our current model for J37015 we ought to get a similar curve as for the AHL levels. To Tom: I remember you saying you've talked to Vincent about the luxI - what was the conclusion?

Revision as of 09:22, 21 August 2006

Purpose of this Page

Tom is away revising for exams and John is going away to the US. This page is a place where questions are raised and answered and a place to keep everyone aware of what is going on.

Monitoring Cell Populations

  • Tom 07:35, 21 August 2006 (EDT): Hi everyone, just been reading through this. Big drawback of the 3 antibiotic way of monitoring cell population that you've identified is the extra ligation steps. What extra ligations steps are involved? If you just mean grafting your parts into a plasmid with appropriate antibiotic markers, can't you just co-transform a seperate plasmid with the marker on, thus saving a ligation step? I'm pretty sure those kind of plasmids would be easy to get hold of as they are the basis of a lot of gene cloning.
    • Tom 10:03, 21 August 2006 (EDT): Actually, on second thoughts, that wouldn't work, as you'll end up losing the plasmid containing the antibiotic you're not screening for while the cells are growing
  • Johnsy 11:30, 21 August 2006 (EDT): From what I understand from the team, we will use the antibiotic way of monitoring as a backup plan, in case we decide to monitor cell populations. For now, we have not decided to monitor cell populations, but we will go ahead and electroporate & Maxi the parts we might need. Just in case...

Measuring LuxI

  • Farah 12:22, 21 August 2006 (EDT): What are we doing about luxI in J37015? Are we going ahead with measuring the GFP levels in that part? Tom mentioned talking to Vincent about it...I can model the luxI but from the assumptions we've made for our current model for J37015 we ought to get a similar curve as for the AHL levels. To Tom: I remember you saying you've talked to Vincent about the luxI - what was the conclusion?
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