IGEM:IMPERIAL/2006/The Talk Page

Purpose of this Page

Tom is away revising for exams and John is going away to the US. This page is a place where questions are raised and answered and a place to keep everyone aware of what is going on.

Monitoring Cell Populations

• Tom 07:35, 21 August 2006 (EDT): Hi everyone, just been reading through this. Big drawback of the 3 antibiotic way of monitoring cell population that you've identified is the extra ligation steps. What extra ligations steps are involved? If you just mean grafting your parts into a plasmid with appropriate antibiotic markers, can't you just co-transform a seperate plasmid with the marker on, thus saving a ligation step? I'm pretty sure those kind of plasmids would be easy to get hold of as they are the basis of a lot of gene cloning.