IGEM:IMPERIAL/2006/project/Bio elec interface

Biological to Electrical Interface

System level diagram of biological to electrical interface

Specifications

Bio Reporter	Signal Transducer
Expressed by E.coli	Detect Bio reporter
Degradation in medium afterwards. Time needed for degradation should be equal to/less than that of GFP	Specific response to bio reporter only
No other interaction with E.coli processes	Real-time readout
Sufficient expression and secretion of bio reporter in medium for detection by signal transducer	Sufficient sensitivity to detect changes in bio reporter
	Commercially available

Possible Implementation

1. Signal transduction could involve (protein) redox-reactions
2. Antigen/Antibody sensors?
3. Protein sensors
4. Hormone/Neurotransmitter sensors (e.g. serotonin)

Scenario 1:

• E.coli is in a liquid medium
• Some bioprocess triggers production of a bio reporter (protein) in E.coli
• The bio reporter is expelled from the cell into the medium
• A sensor will measure the level of bio reporter (protein) in the medium

Scenario 2:

• E.coli is in a liquid medium
• Some bioprocess triggers productio of a bioreporter (protein) in E.coli
• The bio reporter is expelled from the cell into the medium
• There, the bio reporter (protein/enzyme) breaks down a certain chemical present in the medium
• A commercially available sensor then measures the

a) increase in concentration of the breakdown products or,

b) the decrease in concentratio of the initial chemical

Summary of meeting with Professor Tony Cass

Summary of meeting with Dr. Anna Radomska

Further Reading

• pH-based enzyme potentiometric sensors. Part 1. Theory
• pH-based enzyme potentiometric sensors. Part 2. Glucose-sensitive field effect transistor
• pH-based enzyme potentiometric sensors. Part 3. Penicillin-sensitive field effect transistor
• Response of an enzyme-modified pH-sensitive ion selective device; consideration of the influence of the buffering capacity of the analyte solution
• Response of an enzyme-modified pH-sensitive ion-selective device; experimental study of a glucose oxidase-modified ion-sensitive field effect transistor in buffered and unbuffered aqueous solution
• Response of an enzyme-modified pH-sensitive ion-selective device; analytical solution for the response in the presence of pH buffer