IGEM:IMPERIAL/2006/project/Oscillator/Modelling/Realistic Values: Difference between revisions

Revision as of 02:12, 16 August 2006

Gene expresion: 2 proteins/sec/plasmid
- Source: Modeling the Lux/AiiA Relaxation Oscillator

LuxR Half-Life: 60min
- Can be varied between 40 and 100min using furones
- Source: Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover

AHL Half-Life: 3 Hours (Conflicting information)
- Source: Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus
- Conflict: Cell-Cell Communication by Quorum Sensing sugests AHL half-life is 24 hours

aiiA Half-Life: 24hrs
- Could be halved by degredation tagging
- Source: Dr Mann

LuxI Vmax: 1.1mol HSL/mol LuxI/min
- Can be increased by over a factor of at least 1000 with addition of SAM and hexanoyl-ACP
- Source: Acyl-homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein
- Source: Cell-Cell Communication by Quorum Sensing

LuxI Km:
- 1. For hexanoyl-ACP: 9.6uM
- 2. For SAM: 130uM
- Source: Acyl-homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein

aiiA Vmax: 68.6 nmol/min per mg = 1.715mol/min per mol
- Source: The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase

aiiA Km: 0.74mM
- Source: The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase

@@ Line 8: / Line 8: @@
 **Source: [http://mic.sgmjournals.org/cgi/reprint/148/4/1119 Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover]
-*<font color=green>'''HSL Half-Life:'''</font> 3 Hours <font color=red>'''(Conflicting information)'''</font>
+*<font color=green>'''AHL Half-Life:'''</font> 3 Hours <font color=red>'''(Conflicting information)'''</font>
 **Source: [http://jb.asm.org/cgi/reprint/182/24/6921 Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus]
 **Conflict: [http://155.198.83.35/igemwiki2006/mediawiki-1.6.6/images/7/71/Cell-Cell_Communication_by_Quorum_Sensing.pdf Cell-Cell Communication by Quorum Sensing] sugests AHL half-life is '''24 hours'''