IGEM:IMPERIAL/2006/project/Oscillator/Problems/Preycells

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The prey cells don't flouressce! This is a majour problem!!! what are we doing about it?

  • This data suggests that the prey cells are flourescing however there is no propper controll so the experiment needs to be repeated [1] I am pretty sure the increaced flourescence is due to the presence of cells not producing GFP. There needs to be a positive controll.
  • My experiment yesterday showed that the prey cells DO NOT PRODUCE AHL! this is a big problem

How can we fix this?

  • Tom 14:10, 26 August 2006 (EDT): When I did the initial testing of J37015 I initally got a AHL and flourescence flatline. However, repeating the experiment and missing out the dilution steps from the o/n culture I got this output for AHL (although I forgot to measure J37015 direct flouresence, oops!). The data does show that AHL is being produced.
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