IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Quality control for the prey part] | *[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Quality control for the prey part] | ||
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Quality control for the predator part] | *[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Quality control for the predator part] | ||
==Implementation== | |||
*The oscillator will be tested in a [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation/chemostat|chemostat]]. This equipment works to: | |||
::*Keep the cells in the exponential growth phase | |||
::*Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio | |||
::*Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat | |||
==WetLab work entries== | ==WetLab work entries== | ||
Revision as of 20:20, 29 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Prey Molecule Generator | Predator Molecule Generator |
Ligation strategy
Quality control
Implementation
- The oscillator will be tested in a chemostat. This equipment works to:
- Keep the cells in the exponential growth phase
- Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
- Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat
WetLab work entries
- The prey has been constructed and tested but is waiting parameter extraction.
- The predator construct has not been ligated.
- Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
- We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.
- FV:To finish page:
- Need sequencing results for J37023 and any intermediates
- Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.
