IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions

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|-
|-
!height="25pt" width="80pt"|Actual Part
!height="25pt" width="80pt"|Actual Part
!colspan="2"| [[Image:J37036 logo.png]]
!colspan="2"| [[Image:J37036 part.png]]
|-
|-
!height="25pt" width="80pt"|Sub Parts
!height="25pt" width="80pt"|Sub Parts
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey_Construct| Prey Construct]]  
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey_Construct| Prey Molecule Generator]]  
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator_Construct| Predator Construct]]  
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator_Construct| Predator Molecule Generator]]  
|}
|}
<div class="tabs-blue">
<div class="tabs-blue">
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==Ligation strategy==
==Ligation strategy==


*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Ligations for the prey]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Ligations for the predator]




*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].


<u><font size="2">'''Ligating the Prey Construct:'''</font></u>
==Quality control==
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2
|-
!style="background:#D9F2ED"|<center><bbpart>J37034</bbpart>=<br>(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:C0261 logo.png]][[Image:Plus sign.png|20px]][[Image:I13504 logo.png]]</center>
|-
!style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center>
|}


<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u>
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Quality control for the prey part]
__NOTOC__
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Quality control for the predator part]
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4
|-
!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> || ||  ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:Plus sign.png|20px]][[Image:J37024 logo.png]]</center> || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)</center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:J37024 logo.png]]</center> ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center> || || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png]][[Image:C0062 logo.png]]</center> || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37036</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)</center>|| ||  || !!style="background:#CCEBAD" | <center>[[Image:J37036 logo.png]]</center>
|}


<u><font size="2">'''Ligating the Monocystronic Predator Construct:'''</font></u>
==Implementation==
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4 !! style="background:#E6B3E0" |Stage 5
|-
!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]]</center> || || || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:J37024 logo.png|70px]]</center> || || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)</center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center> || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png|30px]]</center> || || || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]]</center> || || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37031</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)</center>|| || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0014 logo.png|50px]]
|-
!style="background:#D9F2ED"|<center><bbpart>J37018</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)</center>||  ||  || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:Plus sign.png|20px]][[Image:R0062 logo.png|30px]]</center>
|-
!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center>
|}


==Quality control==
*The oscillator will be tested in a [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation/chemostat|chemostat]]. This equipment works to:
__NOTOC__
::*Keep the cells in the exponential growth phase
{| border="1" cellpadding="0" width="100%"
::*Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
|-
::*Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequencing Results !! Primers Used for Sequencing
|-
| <center><bbpart>J37018</bbpart></center> || [[Image:J37018 logo.png]] || No || N/A || primers used
|-
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || ghfhjgjh || primers used
|-
| <center><bbpart>J37023</bbpart></center> || [[Image:J37015 part.png|450px]]  || Yes || guyuytg || primers used
|-
| <center><bbpart>J37024</bbpart></center> || [[Image:J37036 part.png]] || No || N/A || primers used
|-
| <center><bbpart>J37025</bbpart></center> || [[Image:J37015 part.png|450px]]  || No || N/A || primers used
|-
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || No || N/A || primers used
|-
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || ghfg || primers used
|-
| <center><bbpart>J37035</bbpart></center> || [Image:J37036 part.png]] || No || N/A || primers used
|-
| <center><bbpart>J37036</bbpart></center> || [[Image:J37015 part.png|450px]] || Yes || jhgjhg || primers used
|}


==WetLab work entries==
==WetLab work entries==
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*The prey has been constructed and tested but is waiting parameter extraction.
*The prey has been constructed and tested but is waiting parameter extraction.
*The predator construct has not been ligated.
*The predator construct has not been ligated.
::*Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
::*We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.
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Latest revision as of 09:39, 30 October 2006

Super Parts Not applicable
Actual Part
Sub Parts Prey Molecule Generator Predator Molecule Generator


Ligation strategy


Quality control

Implementation

  • The oscillator will be tested in a chemostat. This equipment works to:
  • Keep the cells in the exponential growth phase
  • Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
  • Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat

WetLab work entries

  • The prey has been constructed and tested but is waiting parameter extraction.
  • The predator construct has not been ligated.
  • Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
  • We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.

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