IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions

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==Ligation strategy==
==Ligation strategy==


*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Ligations for the prey]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Ligations for the prey]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Ligations for the predator]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Ligations for the predator]
 
 
*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].


==Quality control==
==Quality control==
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Quality control for the prey part]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Quality control for the prey part]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Quality control for the predator part]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Quality control for the predator part]
==Implementation==
*The oscillator will be tested in a [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation/chemostat|chemostat]]. This equipment works to:
::*Keep the cells in the exponential growth phase
::*Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
::*Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat


==WetLab work entries==
==WetLab work entries==
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::*We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.
::*We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.


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*<font color=red>FV:'''To finish page:'''
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::*Need sequencing results for J37023 and any intermediates
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::*Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.</font>
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Latest revision as of 09:39, 30 October 2006

Super Parts Not applicable
Actual Part
Sub Parts Prey Molecule Generator Predator Molecule Generator


Ligation strategy


Quality control

Implementation

  • The oscillator will be tested in a chemostat. This equipment works to:
  • Keep the cells in the exponential growth phase
  • Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
  • Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat

WetLab work entries

  • The prey has been constructed and tested but is waiting parameter extraction.
  • The predator construct has not been ligated.
  • Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
  • We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.

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