IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions
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==Ligation strategy== | ==Ligation strategy== | ||
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/ | *[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Ligations for the prey] | ||
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/ | *[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Ligations for the predator] | ||
*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page]. | *More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page]. | ||
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::*Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon. | ::*Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon. | ||
::*We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene. | ::*We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene. | ||
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Latest revision as of 09:39, 30 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Prey Molecule Generator | Predator Molecule Generator |
Ligation strategy
- More information on ligations can be found on the Ligation Page.
Quality control
Implementation
- The oscillator will be tested in a chemostat. This equipment works to:
- Keep the cells in the exponential growth phase
- Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
- Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat
WetLab work entries
- The prey has been constructed and tested but is waiting parameter extraction.
- The predator construct has not been ligated.
- Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
- We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.
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