IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions
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!style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center> | !style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center> | ||
|} | |} | ||
<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u> | <u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u> | ||
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!style="background:#D9F2ED"|<center><bbpart>J37036</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || !!style="background:#CCEBAD" | <center>[[Image:J37036 logo.png]]</center> | !style="background:#D9F2ED"|<center><bbpart>J37036</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || !!style="background:#CCEBAD" | <center>[[Image:J37036 logo.png]]</center> | ||
|} | |} | ||
<u><font size="2">'''Ligating the Monocystronic Predator Construct:'''</font></u> | <u><font size="2">'''Ligating the Monocystronic Predator Construct:'''</font></u> | ||
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!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center> | !style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center> | ||
|} | |} | ||
==Quality control== | ==Quality control== | ||
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| <center><bbpart>J37036</bbpart></center> || [[Image:J37015 part.png|450px]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font> | | <center><bbpart>J37036</bbpart></center> || [[Image:J37015 part.png|450px]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font> | ||
|} | |} | ||
==WetLab work entries== | ==WetLab work entries== | ||
Revision as of 19:06, 28 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Prey Construct | Predator Construct |
Ligation strategy
Ligating the Prey Construct:
| For | Stage 1 | Stage 2 |
|---|---|---|
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>) |
   | |
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>) |
 |
Ligating the Polycystronic Predator Construct:
| For | Stage 1 | Stage 2 | Stage 3 | Stage 4 |
|---|---|---|---|---|
(<bbpart>"PCR'd"</bbpart>) |
 |
|||
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>) |
 |
|||
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>) |
|
|||
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>) |
  |
|||
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>) |
 |
|||
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>) |
|
Ligating the Monocystronic Predator Construct:
| For | Stage 1 | Stage 2 | Stage 3 | Stage 4 | Stage 5 |
|---|---|---|---|---|---|
(<bbpart>"PCR'd"</bbpart>) |
|
||||
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>) |
|
||||
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>) |
|
||||
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>) |
|
||||
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>) |
|
||||
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>) |
| ||||
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>) |
| ||||
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>) |
|
Quality control
| Part | Logo | Built | Sequenced | Primers Used for Sequencing |
|---|---|---|---|---|
 |
No | N/A | N/A | |
 |
Yes | Yes | Forward pSB1(AK3/A3/A2)
Sequence: TAT TGT CTC ATG AGC GG | |
 |
Yes | Yes | ? | |
 |
No | N/A | N/A | |
|
No | N/A | N/A | |
 |
No | N/A | N/A | |
 |
Yes | Not sequenced | N/A | |
 |
No | N/A | N/A | |
|
Yes | ? | ? |
WetLab work entries
- The prey has been constructed and tested but is waiting parameter extraction.
- The predator construct has not been ligated.
- Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
- We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.
