IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation

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(Ligation strategy)
(Quality control)
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!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
|-
|-
-
| <center><bbpart>J37018</bbpart></center> || [[Image:J37018 logo.png]] || No || N/A || N/A
+
| <center><bbpart>J37016</bbpart></center> || [[Image:J37016 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|-
|-
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
Line 97: Line 97:
Seconday Structure: None
Seconday Structure: None
|-
|-
-
| <center><bbpart>J37023</bbpart></center> || [[Image:J37015 part.png|450px]] || Yes || [http://openwetware.org/images/b/b1/J37015_sequencing.doc Yes] || <font color=red>'''?'''</font>
+
| <center><bbpart>J37020</bbpart></center> || [[Image:J37020 logo.png]] || No || N/A || N/A
|-
|-
-
| <center><bbpart>J37024</bbpart></center> || [[Image:J37036 part.png]] || No || N/A || N/A
+
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|-
|-
-
| <center><bbpart>J37025</bbpart></center> || [[Image:J37015 part.png|450px]] || No || N/A || N/A
+
| <center><bbpart>J37032</bbpart></center> || [[Image:J37032 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|-
|-
-
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || No || N/A || N/A
+
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 part.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
-
|-
+
-
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || Not sequenced || N/A
+
-
|-
+
-
| <center><bbpart>J37035</bbpart></center> || [[Image:J37035 logo.png]] || No || N/A || N/A
+
-
|-
+
-
| <center><bbpart>J37036</bbpart></center> || [[Image:J37015 part.png|450px]] || No || N/A || N/A
+
|}
|}

Revision as of 14:03, 29 October 2006

Super Parts Not applicable
Actual Part Image:J37036 part.png
Sub Parts Prey Construct Predator Construct


Ligation strategy

Ligating the Prey Construct:

For Stage 1 Stage 2
<bbpart>J37034</bbpart>=
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)
Image:C0261 logo.pngImage:I13504 logo.png
<bbpart>J37015</bbpart>=
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)
Image:J37015 logo.png


Ligating the Polycystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
Image:J37023 logo.png
<bbpart>J37024</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
Image:B0034 logo.pngImage:J37023 logo.png
<bbpart>J37025</bbpart>=
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
Image:B0034 logo.pngImage:J37024 logo.png
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37036</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37036 logo.png


Ligating the Monocystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4 Stage 5
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>=
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37016</bbpart>
Image:J37016 logo.png Yes ? ?
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Forward pSB1(AK3/A3/A2)

Sequence: TAT TGT CTC ATG AGC GG
Seconday Structure: None

<bbpart>J37020</bbpart>
Image:J37020 logo.png No N/A N/A
<bbpart>J37031</bbpart>
Image:J37031 logo.png Yes ? ?
<bbpart>J37032</bbpart>
Image:J37032 logo.png Yes ? ?
<bbpart>J37033</bbpart>
Image:J37033 part.png Yes ? ?

WetLab work entries

  • The prey has been constructed and tested but is waiting parameter extraction.
  • The predator construct has not been ligated.
  • Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
  • We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.


  • FV:To finish page:
  • Need sequencing results for J37023 and any intermediates
  • Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.
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